Linked Life Data

9711629

Source:http://linkedlifedata.com/resource/pubmed/id/9711629

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:dateCreated
1998-9-4
pubmed:abstractText
Transient transfection is an excellent method for the expression and study of cell-surface, heteromeric ion channels. The cell type, the total amount of DNA, the combination of subunits and the ratio of subunit DNA are all important parameters to consider when attempting to optimize expression. A serious drawback of this method is that the efficiency of subunit assembly is very low in comparison to the efficiency of assembly for stably expressed heteromeric ion channels. The low efficiency of assembly prevents use of transient expression methods for detailed studies of heteromeric AChR assembly, and caution should be taken in the use of these methods for the study of intracellular heteromeric ion channel subunits. After the transient expression of heteromeric AChR subunits, virtually all of the expressing cells contained all four AChR subunits. However, the subunits were heterogeneously distributed among the cells, and the low efficiency of AChR assembly appears to be due to cell-to-cell variations in the ratio of the four subunits.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:issn
0076-6879
pubmed:author
pubmed:issnType
Print
pubmed:volume
293
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
564-85
pubmed:dateRevised
2006-11-15
pubmed:meshHeading
pubmed:year
1998
pubmed:articleTitle
Transient expression of heteromeric ion channels.
pubmed:affiliation
Committee on Neurobiology, University of Chicago, Illinois 60637, USA.
pubmed:publicationType
Journal Article, Research Support, U.S. Gov't, P.H.S., Research Support, Non-U.S. Gov't