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rdf:type |
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lifeskim:mentions |
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pubmed:issue |
9
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pubmed:dateCreated |
1998-9-10
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pubmed:abstractText |
Kaposi's sarcoma-associated herpesvirus (KSHV) is consistently identified in Kaposi's sarcoma and body cavity-based lymphoma. KSHV encodes a transforming protein called K1 which is structurally similar to lymphocyte receptors. We have found that a highly conserved region of the cytoplasmic domain of K1 resembles the sequence of immunoreceptor tyrosine-based activation motifs (ITAMs). To demonstrate the signal-transducing activity of K1, we constructed a chimeric protein in which the cytoplasmic tail of the human CD8alpha polypeptide was replaced with that of KSHV K1. Expression of the CD8-K1 chimera in B cells induced cellular tyrosine phosphorylation and intracellular calcium mobilization upon stimulation with an anti-CD8 antibody. Mutational analyses showed that the putative ITAM of K1 was required for its signal-transducing activity. Furthermore, tyrosine residues of the putative ITAM of K1 were phosphorylated upon stimulation, and this allowed subsequent binding of SH2-containing proteins. These results demonstrate that the KSHV transforming protein K1 contains a functional ITAM in its cytoplasmic domain and that it can transduce signals to induce cellular activation.
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pubmed:grant |
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pubmed:commentsCorrections |
http://linkedlifedata.com/resource/pubmed/commentcorrection/9710606-1705867,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9710606-7511210,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9710606-7561018,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9710606-7664345,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9710606-7680959,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9710606-7684459,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9710606-7700311,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9710606-7834741,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9710606-7859281,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9710606-7895172,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9710606-7941343,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9710606-7997879,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9710606-8262054,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9710606-8293463,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9710606-8306975,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9710606-8334702,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9710606-8612236,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9710606-8642350,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9710606-8808675,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9710606-8939871,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9710606-8962146,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9710606-8985427,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9710606-9002520,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9710606-9032330,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9710606-9055855,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9710606-9055856,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9710606-9087414,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9710606-9151804,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9710606-9151805,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9710606-9234701,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9710606-9287217,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9710606-9299627,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9710606-9365244,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9710606-9371642,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9710606-9420276,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9710606-9546789,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9710606-9620998
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
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pubmed:status |
MEDLINE
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pubmed:month |
Sep
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pubmed:issn |
0270-7306
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pubmed:author |
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pubmed:issnType |
Print
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pubmed:volume |
18
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
5219-28
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pubmed:dateRevised |
2009-11-18
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pubmed:meshHeading |
pubmed-meshheading:9710606-Amino Acid Sequence,
pubmed-meshheading:9710606-Animals,
pubmed-meshheading:9710606-B-Lymphocytes,
pubmed-meshheading:9710606-COS Cells,
pubmed-meshheading:9710606-Calcium,
pubmed-meshheading:9710606-Cell Line,
pubmed-meshheading:9710606-Conserved Sequence,
pubmed-meshheading:9710606-Herpesvirus 8, Human,
pubmed-meshheading:9710606-Humans,
pubmed-meshheading:9710606-Models, Biological,
pubmed-meshheading:9710606-Molecular Sequence Data,
pubmed-meshheading:9710606-Mutagenesis, Site-Directed,
pubmed-meshheading:9710606-Phosphorylation,
pubmed-meshheading:9710606-Receptors, Antigen, T-Cell, alpha-beta,
pubmed-meshheading:9710606-Recombinant Fusion Proteins,
pubmed-meshheading:9710606-Sequence Alignment,
pubmed-meshheading:9710606-Signal Transduction,
pubmed-meshheading:9710606-Transfection,
pubmed-meshheading:9710606-Tyrosine,
pubmed-meshheading:9710606-Viral Proteins
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pubmed:year |
1998
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pubmed:articleTitle |
Identification of an immunoreceptor tyrosine-based activation motif of K1 transforming protein of Kaposi's sarcoma-associated herpesvirus.
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pubmed:affiliation |
Department of Microbiology and Molecular Genetics, New England Regional Primate Research Center, Harvard Medical School, Southborough, Massachusetts 01772, USA.
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pubmed:publicationType |
Journal Article,
Research Support, U.S. Gov't, P.H.S.
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