Linked Life Data

971045

Source:http://linkedlifedata.com/resource/pubmed/id/971045

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
4
pubmed:dateCreated
1976-12-1
pubmed:abstractText
A method is reported for considering tumour-host relationships, which consists in cultivation of human tumours jointly with autologous lymphocytes in an organ culture system and determining separately tumour and lymphocyte DNA synthesis before and after mixed culture. Some experiments explore variables of the new system. For mixed organ cultures the best conditions are: 4-day incubation, lymphocyte DNA ratio to tumour cell DNA less than 9:1 (2-10(6) lymphocytes/ml) and autologous serum. So far 41 tumours (20 mammary, 17 gastric, and 4 bronchial carcinomas) have been examined. 22 tumours showed satisfactory in vitro maintenance. In 50% of the mixed organ cultures, lymphocyte DNA synthesis proved to be higher than in lymphocyte control cultures. Blast counts in the cell smears are in good accord with results obtained by biochemical determination of DNA synthesis. 7 of the tumours studied have shown a pronounced reduction of DNA synthesis in the presence of lymphocytes. The present results underline the individuality of tumour-host relationships and suggest the necessity of determinations of the immune reactivity in cancer patients. Advantages and disadvantages of the method are discussed.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:issn
0003-911X
pubmed:author
pubmed:issnType
Print
pubmed:volume
46
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
281-93
pubmed:dateRevised
2006-11-15
pubmed:meshHeading
pubmed:year
1976
pubmed:articleTitle
Mixed organ culture as a tool for considering cellular tumor-host relationships in tumor patients.
pubmed:publicationType
Journal Article, In Vitro