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| Predicate | Object |
|---|---|
| rdf:type | |
| lifeskim:mentions | |
| pubmed:issue |
3
|
| pubmed:dateCreated |
1998-8-25
|
| pubmed:abstractText |
Proteases are crucial for cancer metastasis, but due to lack of assays, their role in intravasation has not yet been tested. We have developed a human Alu sequence PCR-based assay to quantitate intravasated cells in an in vivo model. We demonstrated that metalloproteinases (MMPs), and most likely MMP-9, are required for intravasation by showing that marimastat, an inhibitor of MMPs, reduced intravasation by more than 90%, and that only tumor cell lines expressing MMP-9 intravasated. Cells with low surface urokinase plasminogen activator (uPA) and uPA receptor (uPAR) were also incapable of intravasation, despite the presence of high levels of MMP-9. We concluded that breaching of the vascular wall is a rate-limiting step for intravasation, and consequently for metastasis, and that cooperation between uPA/uPAR and MMP-9 is required to complete this step.
|
| pubmed:grant | |
| pubmed:language |
eng
|
| pubmed:journal | |
| pubmed:citationSubset |
IM
|
| pubmed:chemical | |
| pubmed:status |
MEDLINE
|
| pubmed:month |
Aug
|
| pubmed:issn |
0092-8674
|
| pubmed:author | |
| pubmed:issnType |
Print
|
| pubmed:day |
7
|
| pubmed:volume |
94
|
| pubmed:owner |
NLM
|
| pubmed:authorsComplete |
Y
|
| pubmed:pagination |
353-62
|
| pubmed:dateRevised |
2007-11-14
|
| pubmed:meshHeading |
pubmed-meshheading:9708737-Allantois,
pubmed-meshheading:9708737-Animals,
pubmed-meshheading:9708737-Cell Membrane,
pubmed-meshheading:9708737-Cell Transformation, Neoplastic,
pubmed-meshheading:9708737-Chick Embryo,
pubmed-meshheading:9708737-Chorion,
pubmed-meshheading:9708737-Endopeptidases,
pubmed-meshheading:9708737-Female,
pubmed-meshheading:9708737-Humans,
pubmed-meshheading:9708737-Hydrolysis,
pubmed-meshheading:9708737-Male,
pubmed-meshheading:9708737-Neoplasm Invasiveness,
pubmed-meshheading:9708737-Neoplasm Metastasis,
pubmed-meshheading:9708737-Polymerase Chain Reaction,
pubmed-meshheading:9708737-Repetitive Sequences, Nucleic Acid,
pubmed-meshheading:9708737-Sensitivity and Specificity,
pubmed-meshheading:9708737-Tumor Cells, Cultured,
pubmed-meshheading:9708737-Vascular Neoplasms
|
| pubmed:year |
1998
|
| pubmed:articleTitle |
Requirement for specific proteases in cancer cell intravasation as revealed by a novel semiquantitative PCR-based assay.
|
| pubmed:affiliation |
Mount Sinai School of Medicine, Division of Neoplastic Diseases, New York, New York 10029, USA.
|
| pubmed:publicationType |
Journal Article,
Research Support, U.S. Gov't, P.H.S.,
Research Support, Non-U.S. Gov't
|