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rdf:type |
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lifeskim:mentions |
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pubmed:issue |
4
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pubmed:dateCreated |
1998-9-16
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pubmed:abstractText |
Reaction of isatoic anhydride with adenosine, adenosine 5'-phosphate, oligoribonucleotides or with the E. coli tRNAVal led to attachment of an anthraniloyl residue at 2'- or 3'-OH groups of 3'-terminal ribose residue. No protection of the 5'-hydroxyl group or internal 2'-hydroxyl groups is required for this specific reaction. Anthraniloyl-tRNA which is an analogue of aminoacyl-tRNA forms a ternary complex with EF-Tu*GTP. The anthraniloyl-residue is used as a fluorescent reporter group to monitor interactions with proteins.
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
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pubmed:status |
MEDLINE
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pubmed:month |
Apr
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pubmed:issn |
0732-8311
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pubmed:author |
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pubmed:issnType |
Print
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pubmed:volume |
17
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
815-29
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pubmed:dateRevised |
2006-11-15
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pubmed:meshHeading |
pubmed-meshheading:9708336-Anthranilic Acids,
pubmed-meshheading:9708336-Base Sequence,
pubmed-meshheading:9708336-Chromatography, Ion Exchange,
pubmed-meshheading:9708336-Electrophoresis, Polyacrylamide Gel,
pubmed-meshheading:9708336-Escherichia coli,
pubmed-meshheading:9708336-Oligoribonucleotides,
pubmed-meshheading:9708336-RNA, Bacterial,
pubmed-meshheading:9708336-RNA, Transfer, Amino Acyl,
pubmed-meshheading:9708336-Spectrometry, Fluorescence
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pubmed:year |
1998
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pubmed:articleTitle |
Aminoacyl-tRNA analogues; synthesis, purification and properties of 3'-anthraniloyl oligoribonucleotides.
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pubmed:affiliation |
Laboratorium für Biochemie, Universität Bayreuth, Germany. bnawrot@lodz.pdi.net
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pubmed:publicationType |
Journal Article,
Research Support, Non-U.S. Gov't
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