Linked Life Data

9705221

Source:http://linkedlifedata.com/resource/pubmed/id/9705221

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
2
pubmed:dateCreated
1998-9-16
pubmed:abstractText
The mRNA expression of human UDP-glucuronosyltransferase 1A8 (UGT1A8) has been found in jejunum, ileum, and colon but not in liver. A cDNA with a complete UGT1A8 coding region was amplified from total human ileal RNA by reverse transcriptase-polymerase chain reaction and inserted into the mammalian expression vector, pcDNA3. Lysates of HK293 cells expressing UGT1A8 revealed the expression of a protein with a molecular mass of 56 kDa by Western blot analysis. Transiently expressed human UGT1A8 shows glucuronidation activities with coumarins, anthraquinones, flavonoids, phenolic compounds, catechol estrogens, 17-hydroxyandrogens, primary amines such as the carcinogen 4-aminobiphenyl, and certain opioids. This UGT may play an important role in the detoxification of xenobiotics in human intestine.
pubmed:grant
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Aug
pubmed:issn
0003-9861
pubmed:author
pubmed:copyrightInfo
Copyright 1998 Academic Press.
pubmed:issnType
Print
pubmed:day
15
pubmed:volume
356
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
301-5
pubmed:dateRevised
2007-11-14
pubmed:meshHeading
pubmed:year
1998
pubmed:articleTitle
Cloning and expression of human UDP-glucuronosyltransferase (UGT) 1A8.
pubmed:affiliation
Department of Pharmacology, The University of Iowa, Iowa City, Iowa, 52242, USA.
pubmed:publicationType
Journal Article, Research Support, U.S. Gov't, P.H.S.