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| Predicate | Object |
|---|---|
| rdf:type | |
| lifeskim:mentions | |
| pubmed:issue |
8 Pt 1
|
| pubmed:dateCreated |
1998-8-20
|
| pubmed:abstractText |
We describe a new HPLC method for the simultaneous determination of lactulose and mannitol in urine, in which cation-exchange chromatography and evaporative light-scattering detection are used. The two sugars are orally administered for the estimation of intestinal permeability in children. Samples were purified by solid phase extraction on a C18 cartridge and subsequent addition of anion-exchange resin. Cellobiose may be used as an internal standard. The chromatographic separation was carried out in 16 min at a flow rate of 0.5 mL/min, using deionized water as the mobile phase. Within-run precision (CV) measured at three concentrations was 1.6-2.3% for lactulose and 1.0-1.9% for mannitol. Between-run CVs were 2.1-4.1% and 1.3-2.7% for lactulose and mannitol, respectively. Analytical recovery of both sugar probes was 97-101%. The detection limits (signal-to-noise ratio = 3) were 0.82 mg/L for lactulose and 0.65 mg/L for mannitol. The lactulose/ mannitol ratio in control subjects was 0.024 +/- 0.006; in patients with Crohn's and coeliac diseases in active phase, the ratios were 0.200 +/- 0.082 and 0.072 +/- 0.025, respectively. The method is rapid, simple, and sensitive, and suitable for determination of intestinal permeability in children.
|
| pubmed:language |
eng
|
| pubmed:journal | |
| pubmed:citationSubset |
IM
|
| pubmed:chemical | |
| pubmed:status |
MEDLINE
|
| pubmed:month |
Aug
|
| pubmed:issn |
0009-9147
|
| pubmed:author | |
| pubmed:issnType |
Print
|
| pubmed:volume |
44
|
| pubmed:owner |
NLM
|
| pubmed:authorsComplete |
Y
|
| pubmed:pagination |
1685-91
|
| pubmed:dateRevised |
2006-11-15
|
| pubmed:meshHeading |
pubmed-meshheading:9702956-Administration, Oral,
pubmed-meshheading:9702956-Adolescent,
pubmed-meshheading:9702956-Celiac Disease,
pubmed-meshheading:9702956-Child,
pubmed-meshheading:9702956-Child, Preschool,
pubmed-meshheading:9702956-Chromatography, High Pressure Liquid,
pubmed-meshheading:9702956-Chromatography, Ion Exchange,
pubmed-meshheading:9702956-Crohn Disease,
pubmed-meshheading:9702956-Female,
pubmed-meshheading:9702956-Gastrointestinal Agents,
pubmed-meshheading:9702956-Humans,
pubmed-meshheading:9702956-Infant,
pubmed-meshheading:9702956-Intestinal Absorption,
pubmed-meshheading:9702956-Lactulose,
pubmed-meshheading:9702956-Light,
pubmed-meshheading:9702956-Male,
pubmed-meshheading:9702956-Mannitol,
pubmed-meshheading:9702956-Permeability,
pubmed-meshheading:9702956-Scattering, Radiation,
pubmed-meshheading:9702956-Sensitivity and Specificity
|
| pubmed:year |
1998
|
| pubmed:articleTitle |
Simultaneous HPLC determination with light-scattering detection of lactulose and mannitol in studies of intestinal permeability in pediatrics.
|
| pubmed:affiliation |
Department of Pediatrics, University of Padua, Italy.
|
| pubmed:publicationType |
Journal Article,
Clinical Trial,
Research Support, Non-U.S. Gov't
|