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rdf:type |
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lifeskim:mentions |
|
|
pubmed:issue |
4
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pubmed:dateCreated |
1999-5-19
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|
pubmed:abstractText |
Biotin deoxyuridine 5'-triphosphate (dUTP) and digoxigenin dUTP are the labels most commonly used in comparative genomic hybridization (CGH). The relative infrequent use of direct fluorochrome-labeled nucleotides in CGH is related to the lower sensitivity they provide. Here we report the evaluation of two fluorochrome-conjugated nucleotides that have not been previously used in CGH (lissamine-5-dUTP and fluorescein-N6dATP) and show that this direct label combination performs at least as well as the indirect biotin/digoxigenin pair.
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pubmed:language |
eng
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|
pubmed:journal |
|
|
pubmed:citationSubset |
IM
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|
pubmed:chemical |
|
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pubmed:status |
MEDLINE
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pubmed:month |
Aug
|
|
pubmed:issn |
0196-4763
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|
pubmed:author |
|
|
pubmed:issnType |
Print
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|
pubmed:day |
1
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|
pubmed:volume |
32
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
337-41
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pubmed:dateRevised |
2006-11-15
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pubmed:meshHeading |
pubmed-meshheading:9701403-DNA,
pubmed-meshheading:9701403-DNA, Neoplasm,
pubmed-meshheading:9701403-Female,
pubmed-meshheading:9701403-Fluorescein,
pubmed-meshheading:9701403-Genomic Library,
pubmed-meshheading:9701403-Humans,
pubmed-meshheading:9701403-Lymphocytes,
pubmed-meshheading:9701403-Male,
pubmed-meshheading:9701403-Nucleic Acid Hybridization,
pubmed-meshheading:9701403-Nucleotides,
pubmed-meshheading:9701403-Rhodamines
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pubmed:year |
1998
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|
pubmed:articleTitle |
Comparative genomic hybridization with lissamine- and fluorescein-labeled nucleotides.
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pubmed:affiliation |
Department of Molecular Cell Biology, Leiden University Medical Center, The Netherlands. carla@ruly46.leidenuniv.nl
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pubmed:publicationType |
Journal Article,
Comparative Study,
Research Support, Non-U.S. Gov't
|
