Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
2 Pt 1
pubmed:dateCreated
1998-9-16
pubmed:abstractText
We determined whether human lung fibroblasts (HLFs) might release mediators that are responsible for monocyte chemokinetic activity (MCA) constitutively. HLF supernatant fluids showed MCA in a time-dependent manner (P < 0.001). Checkerboard analysis of 24- and 72-h supernatant fluids showed that the activity was chemokinetic. Partial characterization of 24- and 72-h supernatant fluids revealed that the mediators released after 24 h were predominantly composed of lipid-soluble activity, and MCA was blocked by lipoxygenase inhibitors. The mediators released after 72 h were predominantly trypsin sensitive and blocked by cycloheximide. Molecular-sieve column chromatography identified four peaks of MCA. A polyclonal antibody to monocyte chemoattractant protein-1 (MCP-1) inhibited MCA by 20% after 24 h and by 40% after 72 h. Granulocyte-macrophage colony-stimulating factor (GM-CSF) and transforming growth factor-beta (TGF-beta) antibodies attenuated MCA released after 72 h by 30 and 10%, respectively. These antibodies inhibited corresponding molecular-weight peaks separated by molecular-sieve column. The concentrations of MCP-1, GM-CSF, and TGF-beta were 4,698 +/- 242, 26.8 +/- 3.8, and 550 +/- 15 pg/ml, respectively. A leukotriene B4 (LTB4)-receptor antagonist attenuated the total MCA and the lowest molecular weight peak of MCA. The concentrations of LTB4 were 153.4 +/- 12.4 (24 h) and 212 +/- 16.6 (72 h) pg/ml. These findings suggest that HLFs may modulate the recruitment of monocytes into the lung by releasing MCP-1, GM-CSF, TGF-beta, and LTB4 constitutively.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
http://linkedlifedata.com/resource/pubmed/chemical/Antibodies, http://linkedlifedata.com/resource/pubmed/chemical/Chemokine CCL2, http://linkedlifedata.com/resource/pubmed/chemical/Chemokine CCL5, http://linkedlifedata.com/resource/pubmed/chemical/Cycloheximide, http://linkedlifedata.com/resource/pubmed/chemical/Diethylcarbamazine, http://linkedlifedata.com/resource/pubmed/chemical/Granulocyte-Macrophage..., http://linkedlifedata.com/resource/pubmed/chemical/Lipoxygenase Inhibitors, http://linkedlifedata.com/resource/pubmed/chemical/Nordihydroguaiaretic Acid, http://linkedlifedata.com/resource/pubmed/chemical/Platelet Activating Factor, http://linkedlifedata.com/resource/pubmed/chemical/Receptors, Leukotriene B4, http://linkedlifedata.com/resource/pubmed/chemical/Transforming Growth Factor beta, http://linkedlifedata.com/resource/pubmed/chemical/Trypsin
pubmed:status
MEDLINE
pubmed:month
Aug
pubmed:issn
0002-9513
pubmed:author
pubmed:issnType
Print
pubmed:volume
275
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
L223-30
pubmed:dateRevised
2007-11-15
pubmed:meshHeading
pubmed-meshheading:9700081-Adult, pubmed-meshheading:9700081-Antibodies, pubmed-meshheading:9700081-Cell Line, pubmed-meshheading:9700081-Chemokine CCL2, pubmed-meshheading:9700081-Chemokine CCL5, pubmed-meshheading:9700081-Chemotaxis, Leukocyte, pubmed-meshheading:9700081-Cycloheximide, pubmed-meshheading:9700081-Diethylcarbamazine, pubmed-meshheading:9700081-Fibroblasts, pubmed-meshheading:9700081-Granulocyte-Macrophage Colony-Stimulating Factor, pubmed-meshheading:9700081-Humans, pubmed-meshheading:9700081-Kinetics, pubmed-meshheading:9700081-Lipoxygenase Inhibitors, pubmed-meshheading:9700081-Lung, pubmed-meshheading:9700081-Monocytes, pubmed-meshheading:9700081-Nordihydroguaiaretic Acid, pubmed-meshheading:9700081-Platelet Activating Factor, pubmed-meshheading:9700081-Receptors, Leukotriene B4, pubmed-meshheading:9700081-T-Lymphocytes, pubmed-meshheading:9700081-Time Factors, pubmed-meshheading:9700081-Transforming Growth Factor beta, pubmed-meshheading:9700081-Trypsin
pubmed:year
1998
pubmed:articleTitle
Human lung fibroblasts release chemokinetic activity for monocytes constitutively.
pubmed:affiliation
Shinshu University School of Medicine, First Department of Internal Medicine, Matsumoto 390, Japan.
pubmed:publicationType
Journal Article, In Vitro