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rdf:type |
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lifeskim:mentions |
umls-concept:C0004793,
umls-concept:C0011311,
umls-concept:C0017428,
umls-concept:C0020792,
umls-concept:C0042776,
umls-concept:C0205369,
umls-concept:C0332307,
umls-concept:C0598312,
umls-concept:C1514873,
umls-concept:C1546857,
umls-concept:C1556066,
umls-concept:C1619636
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pubmed:issue |
9
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pubmed:dateCreated |
1998-9-16
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pubmed:abstractText |
The flavivirus genome is a positive-stranded approximately 11-kb RNA including 5' and 3' noncoding regions (NCR) of approximately 100 and 400 to 600 nucleotides (nt), respectively. The 3' NCR contains adjacent, thermodynamically stable, conserved short and long stem-and-loop structures (the 3'-SL), formed by the 3'-terminal approximately 100 nt. The nucleotide sequences within the 3'-SL are not well conserved among species. We examined the requirement for the 3'-SL in the context of dengue virus type 2 (DEN2) replication by mutagenesis of an infectious cDNA copy of a DEN2 genome. Genomic full-length RNA was transcribed in vitro and used to transfect monkey kidney cells. A substitution mutation, in which the 3'-terminal 93 nt constituting the wild-type (wt) DEN2 3'-SL sequence were replaced by the 96-nt sequence of the West Nile virus (WN) 3'-SL, was sublethal for virus replication. An analysis of the growth phenotypes of additional mutant viruses derived from RNAs containing DEN2-WN chimeric 3'-SL structures suggested that the wt DEN2 nucleotide sequence forming the bottom half of the long stem and loop in the 3'-SL was required for viability. One 7-bp substitution mutation in this domain resulted in a mutant virus that grew well in monkey kidney cells but was severely restricted in cultured mosquito cells. In contrast, transpositions of and/or substitutions in the wt DEN2 nucleotide sequence in the top half of the long stem and in the short stem and loop were relatively well tolerated, provided the stem-loop secondary structure was conserved.
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pubmed:commentsCorrections |
http://linkedlifedata.com/resource/pubmed/commentcorrection/9696848-1433509,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9696848-1660836,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9696848-1907891,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9696848-2005794,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9696848-2174669,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9696848-2449095,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9696848-2714651,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9696848-3011975,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9696848-3016981,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9696848-3327519,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9696848-3727403,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9696848-3820298,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9696848-3839464,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9696848-4023707,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9696848-7637011,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9696848-7745733,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9696848-7871753,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9696848-8146129,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9696848-8388482,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9696848-8474174,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9696848-8607261,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9696848-8648730,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9696848-8672458,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9696848-8709254,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9696848-8709255,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9696848-9094618,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9696848-9188607,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9696848-9261361
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
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pubmed:status |
MEDLINE
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pubmed:month |
Sep
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pubmed:issn |
0022-538X
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pubmed:author |
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pubmed:issnType |
Print
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pubmed:volume |
72
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
7510-22
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pubmed:dateRevised |
2011-11-17
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pubmed:meshHeading |
pubmed-meshheading:9696848-Animals,
pubmed-meshheading:9696848-Automatic Data Processing,
pubmed-meshheading:9696848-Base Sequence,
pubmed-meshheading:9696848-Cell Line,
pubmed-meshheading:9696848-Conserved Sequence,
pubmed-meshheading:9696848-Dengue Virus,
pubmed-meshheading:9696848-Genome, Viral,
pubmed-meshheading:9696848-Kinetics,
pubmed-meshheading:9696848-Macaca mulatta,
pubmed-meshheading:9696848-Molecular Sequence Data,
pubmed-meshheading:9696848-Mutagenesis,
pubmed-meshheading:9696848-Nucleic Acid Conformation,
pubmed-meshheading:9696848-Phenotype,
pubmed-meshheading:9696848-RNA, Viral,
pubmed-meshheading:9696848-Structure-Activity Relationship,
pubmed-meshheading:9696848-Viral Plaque Assay,
pubmed-meshheading:9696848-Viral Proteins,
pubmed-meshheading:9696848-Virus Replication,
pubmed-meshheading:9696848-West Nile virus
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pubmed:year |
1998
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pubmed:articleTitle |
Identification of specific nucleotide sequences within the conserved 3'-SL in the dengue type 2 virus genome required for replication.
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pubmed:affiliation |
Laboratory of Vector-Borne Virus Diseases, Division of Viral Products, Center for Biologics Evaluation and Research, Food and Drug Administration, Bethesda, Maryland, USA.
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pubmed:publicationType |
Journal Article,
Research Support, U.S. Gov't, Non-P.H.S.,
Research Support, Non-U.S. Gov't
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