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rdf:type |
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lifeskim:mentions |
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pubmed:issue |
4
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pubmed:dateCreated |
1998-10-19
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pubmed:abstractText |
Immunization with specific tumor-associated antigen (Ag) (TAA)-pulsed dendritic cells (DC) has proven to be efficacious in a variety of animal models and is being investigated for the treatment of cancer patients. Use of DC pulsed with specific peptides or transfected with TAA genes has been a focused area of investigation for the induction of potent tumor and viral immune responses. In this study we demonstrate transgene expression, including expression of the MART-1 gene, in DC transfected with plasmid DNA and cationic liposome complexes. These transiently transfected DC, derived from healthy donor monocytes cultured with granulocyte macrophage colony-stimulating factor and interleukin-4, express the transgene and can stimulate naive CD8+ T cells to elicit an antitumor immune response. These cytotoxic T lymphocytes (CTL) were capable of recognizing both known and unknown TAA epitopes and were able to exhibit cytolytic activity against human histocompatibility leukocyte Ag-matched tumor cells expressing the Ag. In addition to their cytolytic function, the CTL displayed an oligoclonal T-cell receptor repertoire, indicating that the presented Ag induced alterations in the T-cell population. The ability to induce tumor-specific CTL in vitro using gene-modified DC transiently expressing TAAs demonstrates the potential use of these Ag-presenting cells to generate future in vivo cancer vaccine strategies.
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
http://linkedlifedata.com/resource/pubmed/chemical/Antigens, Neoplasm,
http://linkedlifedata.com/resource/pubmed/chemical/Carcinoembryonic Antigen,
http://linkedlifedata.com/resource/pubmed/chemical/Chloramphenicol O-Acetyltransferase,
http://linkedlifedata.com/resource/pubmed/chemical/Granulocyte-Macrophage...,
http://linkedlifedata.com/resource/pubmed/chemical/Interleukin-4,
http://linkedlifedata.com/resource/pubmed/chemical/Liposomes,
http://linkedlifedata.com/resource/pubmed/chemical/MART-1 Antigen,
http://linkedlifedata.com/resource/pubmed/chemical/MLANA protein, human,
http://linkedlifedata.com/resource/pubmed/chemical/Neoplasm Proteins,
http://linkedlifedata.com/resource/pubmed/chemical/Receptors, Antigen, T-Cell,
http://linkedlifedata.com/resource/pubmed/chemical/Recombinant Proteins
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pubmed:status |
MEDLINE
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pubmed:issn |
0929-1903
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pubmed:author |
pubmed-author:AltersSS,
pubmed-author:AnY QYQ,
pubmed-author:AshtonJJ,
pubmed-author:BrunettiAA,
pubmed-author:GadeaJJ,
pubmed-author:LebkowskiJJ,
pubmed-author:O'DonoghueGG,
pubmed-author:OkarmaTT,
pubmed-author:PhilipMM,
pubmed-author:PhilipRR,
pubmed-author:SorichMM
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pubmed:issnType |
Print
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pubmed:volume |
5
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
236-46
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pubmed:dateRevised |
2010-11-18
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pubmed:meshHeading |
pubmed-meshheading:9694075-Antigens, Neoplasm,
pubmed-meshheading:9694075-CD8-Positive T-Lymphocytes,
pubmed-meshheading:9694075-Carcinoembryonic Antigen,
pubmed-meshheading:9694075-Cells, Cultured,
pubmed-meshheading:9694075-Chloramphenicol O-Acetyltransferase,
pubmed-meshheading:9694075-Dendritic Cells,
pubmed-meshheading:9694075-Dependovirus,
pubmed-meshheading:9694075-Genes, MHC Class I,
pubmed-meshheading:9694075-Granulocyte-Macrophage Colony-Stimulating Factor,
pubmed-meshheading:9694075-Humans,
pubmed-meshheading:9694075-Interleukin-4,
pubmed-meshheading:9694075-Liposomes,
pubmed-meshheading:9694075-MART-1 Antigen,
pubmed-meshheading:9694075-Major Histocompatibility Complex,
pubmed-meshheading:9694075-Monocytes,
pubmed-meshheading:9694075-Neoplasm Proteins,
pubmed-meshheading:9694075-Receptors, Antigen, T-Cell,
pubmed-meshheading:9694075-Recombinant Proteins,
pubmed-meshheading:9694075-T-Lymphocytes, Cytotoxic,
pubmed-meshheading:9694075-Transfection,
pubmed-meshheading:9694075-Transgenes
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pubmed:articleTitle |
Transgene expression in dendritic cells to induce antigen-specific cytotoxic T cells in healthy donors.
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pubmed:affiliation |
RPR Gencell, Santa Clara, California 95054, USA. ramila.philip@rp-rorer.com
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pubmed:publicationType |
Journal Article
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