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rdf:type |
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lifeskim:mentions |
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pubmed:issue |
17
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pubmed:dateCreated |
1998-10-14
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pubmed:abstractText |
Inhibition of fibroblast growth factor (FGF) signaling prevents trunk and tail formation in Xenopus and zebrafish embryos. While the T-box transcription factor Brachyury (called No Tail in zebrafish) is a key mediator of FGF signaling in the notochord and tail, the pathways activated by FGF in non-notochordal trunk mesoderm have been uncertain. Previous studies have shown that the spadetail gene is required for non-notochordal trunk mesoderm formation; spadetail mutant embryos have major trunk mesoderm deficiencies, but relatively normal tail and notochord development. We demonstrate here that spadetail encodes a T-box transcription factor with homologues in Xenopus and chick. Spadetail is likely to be a key mediator of FGF signaling in trunk non-notochordal mesoderm, since spadetail expression is regulated by FGF signaling. Trunk and tail development are therefore dependent upon the complementary actions of two T-box genes, spadetail and no tail. We show that the regulatory hierarchy among spadetail, no tail and a third T-box gene, tbx6, are substantially different during trunk and tail mesoderm formation, and propose a genetic model that accounts for the regional phenotypes of spadetail and no tail mutants.
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pubmed:grant |
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
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pubmed:status |
MEDLINE
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pubmed:month |
Sep
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pubmed:issn |
0950-1991
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pubmed:author |
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pubmed:issnType |
Print
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pubmed:volume |
125
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
3379-88
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pubmed:dateRevised |
2007-11-14
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pubmed:meshHeading |
pubmed-meshheading:9693141-Amino Acid Sequence,
pubmed-meshheading:9693141-Animals,
pubmed-meshheading:9693141-Base Sequence,
pubmed-meshheading:9693141-Cloning, Molecular,
pubmed-meshheading:9693141-DNA Primers,
pubmed-meshheading:9693141-DNA-Binding Proteins,
pubmed-meshheading:9693141-Fetal Proteins,
pubmed-meshheading:9693141-Fibroblast Growth Factors,
pubmed-meshheading:9693141-Gene Expression Regulation, Developmental,
pubmed-meshheading:9693141-In Situ Hybridization,
pubmed-meshheading:9693141-Mesoderm,
pubmed-meshheading:9693141-Models, Genetic,
pubmed-meshheading:9693141-Molecular Sequence Data,
pubmed-meshheading:9693141-Mutation,
pubmed-meshheading:9693141-Phenotype,
pubmed-meshheading:9693141-Polymerase Chain Reaction,
pubmed-meshheading:9693141-Sequence Homology, Amino Acid,
pubmed-meshheading:9693141-Signal Transduction,
pubmed-meshheading:9693141-T-Box Domain Proteins,
pubmed-meshheading:9693141-Tail,
pubmed-meshheading:9693141-Transcription Factors,
pubmed-meshheading:9693141-Vertebrates,
pubmed-meshheading:9693141-Zebrafish,
pubmed-meshheading:9693141-Zebrafish Proteins
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pubmed:year |
1998
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pubmed:articleTitle |
Molecular identification of spadetail: regulation of zebrafish trunk and tail mesoderm formation by T-box genes.
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pubmed:affiliation |
Department of Biochemistry, Box 357350, University of Washington, Seattle, Washington 98195-7350, USA.
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pubmed:publicationType |
Journal Article,
Research Support, U.S. Gov't, P.H.S.,
Research Support, U.S. Gov't, Non-P.H.S.,
Research Support, Non-U.S. Gov't
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