Linked Life Data

9693037

Source:http://linkedlifedata.com/resource/pubmed/id/9693037

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
1
pubmed:dateCreated
1998-9-14
pubmed:databankReference
pubmed:abstractText
This paper describes the cDNA cloning, genomic organization, and expression of the human RTN2 gene on chromosome 19q13.3, which was recognized by virtue of its high similarity with the human RTN1 (formerly called NSP) gene on chromosome 14q21-q22. In a region of about 12 kb in total, 11 RTN2 exons could be identified. Like the RTN1 gene, the RTN2 gene is transcribed into different mRNA variants. Two have a size of about 2.3 kb, and a third has a size of about 1. 3 kb. The two 2.3-kb transcripts differ because of alternative splicing of exon 5. Transcription of the 1.3-kb transcript starts presumably from an internal promoter within exon 5. The three mRNAs encode three different proteins, RTN2-A (545 aa), RTN2-B (472 aa), and RTN2-C (205 aa), which share a common carboxy-terminal segment of 201 aa. In this common segment, the homology with the RTN1 proteins, with yet unknown function, is found. Two hydrophobic subregions are present, which are thought to be responsible for the association of the RTN1 and RTN2 proteins with the endoplasmic reticulum. The amino-terminal regions of the RTN2-A and RTN2-B proteins are rich in negatively charged residues and in proline and serine residues and contain multiple potential phosphorylation sites. Analysis of the expression of the RTN2 gene shows differential expression in human tissues with a strikingly high expression of the 1.3-kb transcript in skeletal muscle.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Jul
pubmed:issn
0888-7543
pubmed:author
pubmed:copyrightInfo
Copyright 1998 Academic Press.
pubmed:issnType
Print
pubmed:day
1
pubmed:volume
51
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
98-106
pubmed:dateRevised
2006-11-15
pubmed:meshHeading
pubmed-meshheading:9693037-Alternative Splicing, pubmed-meshheading:9693037-Amino Acid Sequence, pubmed-meshheading:9693037-Base Sequence, pubmed-meshheading:9693037-Chromosome Mapping, pubmed-meshheading:9693037-Chromosomes, Human, Pair 19, pubmed-meshheading:9693037-Cloning, Molecular, pubmed-meshheading:9693037-DNA, Complementary, pubmed-meshheading:9693037-Endoplasmic Reticulum, pubmed-meshheading:9693037-Gene Expression, pubmed-meshheading:9693037-Humans, pubmed-meshheading:9693037-Membrane Proteins, pubmed-meshheading:9693037-Molecular Sequence Data, pubmed-meshheading:9693037-Multigene Family, pubmed-meshheading:9693037-Nerve Tissue Proteins, pubmed-meshheading:9693037-RNA, Messenger, pubmed-meshheading:9693037-Sequence Homology, Amino Acid, pubmed-meshheading:9693037-Tissue Distribution
pubmed:year
1998
pubmed:articleTitle
cDNA cloning, genomic organization, and expression of the human RTN2 gene, a member of a gene family encoding reticulons.
pubmed:affiliation
Center for Human Genetics, University of Louvain and Flanders Interuniversity Institute for Biotechnology, Campus Gasthuisberg, Louvain, B-3000, Belgium.
pubmed:publicationType
Journal Article, Research Support, Non-U.S. Gov't