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rdf:type |
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lifeskim:mentions |
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pubmed:issue |
16
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pubmed:dateCreated |
1998-9-8
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pubmed:abstractText |
Recombinant viral or virus-like particles offer new tools for vaccine development. This study investigated hepatitis B core antigen (HBcAg) capsids and RNA phage Qbeta coats as carriers of a foreign epitope to induce antibody responses in mice. HBcAg capsids were shown to induce T cell-independent (TI) antibodies. We found that these particles behave as antigen-specific TI type 1 (TI-1) Ag comparable to other rigidly structured viruses. When a 5-aa long epitope of the pre-S1 domain of hepatitis B surface antigen (HBsAg) was introduced into the optimal position of the HBc molecule, it also behaved as a TI-1 Ag. Best efficiency of the antibody response to the foreign epitope was achieved by a compensatory deletion after the epitope to retain the regular structure of the HBcAg capsid with a highly repetitive superficial exposition of the foreign epitope. For recombinant Qbeta phage coats, a much more efficient antibody response to the foreign epitope was achieved when the foreign epitope was expressed repetitively on a particulate derivate of Qbeta phage coats. Thus, recombinant virus particles are suitable vaccine carriers for the introduction of foreign B cell epitopes, if precise structural requirements are fulfilled.
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pubmed:commentsCorrections |
http://linkedlifedata.com/resource/pubmed/commentcorrection/9689105-1080258,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9689105-1370083,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9689105-2473123,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9689105-2480917,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9689105-3491425,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9689105-4104294,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9689105-4194660,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9689105-7009404,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9689105-7513919,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9689105-7520465,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9689105-7542855,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9689105-7546399,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9689105-7612238,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9689105-7684473,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9689105-8004680,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9689105-8139044,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9689105-8419335,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9689105-8539616,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9689105-8566036,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9689105-8627153,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9689105-8640864,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9689105-8666525,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9689105-8957664,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9689105-8957665,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9689105-8989426,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9689105-8991286,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9689105-9052786,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9689105-9052787,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9689105-9143688,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9689105-9405667,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9689105-9448302,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9689105-99320
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
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pubmed:status |
MEDLINE
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pubmed:month |
Aug
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pubmed:issn |
0027-8424
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pubmed:author |
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pubmed:issnType |
Print
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pubmed:day |
4
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pubmed:volume |
95
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
9477-81
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pubmed:dateRevised |
2009-11-18
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pubmed:meshHeading |
pubmed-meshheading:9689105-Amino Acid Sequence,
pubmed-meshheading:9689105-Animals,
pubmed-meshheading:9689105-Antibody Formation,
pubmed-meshheading:9689105-B-Lymphocytes,
pubmed-meshheading:9689105-Hepatitis B Surface Antigens,
pubmed-meshheading:9689105-Immunodominant Epitopes,
pubmed-meshheading:9689105-Mice,
pubmed-meshheading:9689105-Mice, Nude,
pubmed-meshheading:9689105-Molecular Sequence Data,
pubmed-meshheading:9689105-Protein Precursors,
pubmed-meshheading:9689105-Recombination, Genetic,
pubmed-meshheading:9689105-Species Specificity,
pubmed-meshheading:9689105-T-Lymphocytes,
pubmed-meshheading:9689105-Virion
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pubmed:year |
1998
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pubmed:articleTitle |
T cell-independent type I antibody response against B cell epitopes expressed repetitively on recombinant virus particles.
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pubmed:affiliation |
Institute of Experimental Immunology, Department of Pathology, University Hospital, Schmelzbergstrasse 12, CH-8091 Zürich, Switzerland.
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pubmed:publicationType |
Journal Article,
Research Support, Non-U.S. Gov't
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