|
rdf:type |
|
|
lifeskim:mentions |
|
|
pubmed:issue |
16
|
|
pubmed:dateCreated |
1998-9-8
|
|
pubmed:abstractText |
A Ca2+-pump ATPase, similar to that in the endoplasmic reticulum, has been located on the outer membrane of rat liver nuclei. The effect of cAMP-dependent protein kinase (PKA) on nuclear Ca2+-ATPase (NCA) was studied by using purified rat liver nuclei. Treatment of isolated nuclei with the catalytic unit of PKA resulted in the phosphorylation of a 105-kDa band that was recognized by antibodies specific for sarcoplasmic reticulum Ca2+-ATPase type 2b. Partial purification and immunoblotting confirmed that the 105-kDa protein band phosphorylated by PKA is NCA. The stoichiometry of phosphorylation was 0.76 mol of phosphate incorporated/mol of partially purified enzyme. Measurement of ATP-dependent 45Ca2+ uptake into purified nuclei showed that PKA phosphorylation enhanced the Ca2+-pumping activity of NCA. We show that PKA phosphorylation of Ca2+-ATPase enhances the transport of 10-kDa fluorescent-labeled dextrans across the nuclear envelope. The findings reported in this paper are consistent with the notion that the crosstalk between the cAMP/PKA- and Ca2+-dependent signaling pathways identified at the cytoplasmic level extends to the nucleus. Furthermore, these data support a function for crosstalk in the regulation of calcium-dependent transport across the nuclear envelope.
|
|
pubmed:commentsCorrections |
http://linkedlifedata.com/resource/pubmed/commentcorrection/9689054-1317870,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9689054-1981673,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9689054-2174556,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9689054-2238044,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9689054-2303497,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9689054-2549856,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9689054-2844797,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9689054-2910849,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9689054-2964872,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9689054-3015674,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9689054-3160706,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9689054-3231093,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9689054-6238959,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9689054-6270081,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9689054-6280588,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9689054-6444416,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9689054-7716515,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9689054-7770065,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9689054-7799952,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9689054-7813612,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9689054-7822421,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9689054-7859285,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9689054-7929371,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9689054-7980512,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9689054-7983062,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9689054-8207083,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9689054-8253746,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9689054-8336722,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9689054-8386159,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9689054-8525380,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9689054-8543012,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9689054-8550605,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9689054-8643446,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9689054-8662932,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9689054-8663079,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9689054-8703198,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9689054-8772386,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9689054-8791595,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9689054-8851662,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9689054-8962094,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9689054-9000075,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9689054-9083074,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9689054-9182531,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9689054-9188473,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9689054-9200614,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9689054-9312057,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9689054-9360942,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9689054-942051,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9689054-9478950,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9689054-9489696
|
|
pubmed:language |
eng
|
|
pubmed:journal |
|
|
pubmed:citationSubset |
IM
|
|
pubmed:chemical |
|
|
pubmed:status |
MEDLINE
|
|
pubmed:month |
Aug
|
|
pubmed:issn |
0027-8424
|
|
pubmed:author |
|
|
pubmed:issnType |
Print
|
|
pubmed:day |
4
|
|
pubmed:volume |
95
|
|
pubmed:owner |
NLM
|
|
pubmed:authorsComplete |
Y
|
|
pubmed:pagination |
9178-83
|
|
pubmed:dateRevised |
2010-11-18
|
|
pubmed:meshHeading |
|
|
pubmed:year |
1998
|
|
pubmed:articleTitle |
cAMP-dependent protein kinase phosphorylates and activates nuclear Ca2+-ATPase.
|
|
pubmed:affiliation |
Laboratoire de Neurobiologie Moléculaire des Interactions Cellulaires, UPR 416 du Centre National de la Recherche Scientifique, 5 rue Blaise Pascal, 67084 Strasbourg, France.
|
|
pubmed:publicationType |
Journal Article
|
