Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
8
pubmed:dateCreated
1998-9-24
pubmed:databankReference
http://linkedlifedata.com/resource/pubmed/xref/GENBANK/AF058372, http://linkedlifedata.com/resource/pubmed/xref/GENBANK/AF058373, http://linkedlifedata.com/resource/pubmed/xref/GENBANK/AF058374, http://linkedlifedata.com/resource/pubmed/xref/GENBANK/AF058375, http://linkedlifedata.com/resource/pubmed/xref/GENBANK/AF058376, http://linkedlifedata.com/resource/pubmed/xref/GENBANK/AF058377, http://linkedlifedata.com/resource/pubmed/xref/GENBANK/AF058378, http://linkedlifedata.com/resource/pubmed/xref/GENBANK/AF058379, http://linkedlifedata.com/resource/pubmed/xref/GENBANK/AF058380, http://linkedlifedata.com/resource/pubmed/xref/GENBANK/AF058381, http://linkedlifedata.com/resource/pubmed/xref/GENBANK/AF058382, http://linkedlifedata.com/resource/pubmed/xref/GENBANK/AF058383, http://linkedlifedata.com/resource/pubmed/xref/GENBANK/AF058384, http://linkedlifedata.com/resource/pubmed/xref/GENBANK/AF058385, http://linkedlifedata.com/resource/pubmed/xref/GENBANK/AF058386, http://linkedlifedata.com/resource/pubmed/xref/GENBANK/AF058387, http://linkedlifedata.com/resource/pubmed/xref/GENBANK/AF058388, http://linkedlifedata.com/resource/pubmed/xref/GENBANK/AF061574, http://linkedlifedata.com/resource/pubmed/xref/GENBANK/AF067655
pubmed:abstractText
Phylogenetic analysis of 16S ribosomal DNA (rDNA) clones obtained by PCR from uncultured bacteria inhabiting a wide range of environments has increased our knowledge of bacterial diversity. One possible problem in the assessment of bacterial diversity based on sequence information is that PCR is exquisitely sensitive to contaminating 16S rDNA. This raises the possibility that some putative environmental rRNA sequences in fact correspond to contaminant sequences. To document potential contaminants, we cloned and sequenced PCR-amplified 16S rDNA fragments obtained at low levels in the absence of added template DNA. 16S rDNA sequences closely related to the genera Duganella (formerly Zoogloea), Acinetobacter, Stenotrophomonas, Escherichia, Leptothrix, and Herbaspirillum were identified in contaminant libraries and in clone libraries from diverse, generally low-biomass habitats. The rRNA sequences detected possibly are common contaminants in reagents used to prepare genomic DNA. Consequently, their detection in processed environmental samples may not reflect environmentally relevant organisms.
pubmed:commentsCorrections
http://linkedlifedata.com/resource/pubmed/commentcorrection/9687486-1931012, http://linkedlifedata.com/resource/pubmed/commentcorrection/9687486-2156164, http://linkedlifedata.com/resource/pubmed/commentcorrection/9687486-2296286, http://linkedlifedata.com/resource/pubmed/commentcorrection/9687486-2466341, http://linkedlifedata.com/resource/pubmed/commentcorrection/9687486-2716852, http://linkedlifedata.com/resource/pubmed/commentcorrection/9687486-7535888, http://linkedlifedata.com/resource/pubmed/commentcorrection/9687486-8075799, http://linkedlifedata.com/resource/pubmed/commentcorrection/9687486-8688960, http://linkedlifedata.com/resource/pubmed/commentcorrection/9687486-8799176, http://linkedlifedata.com/resource/pubmed/commentcorrection/9687486-8904410, http://linkedlifedata.com/resource/pubmed/commentcorrection/9687486-8940458, http://linkedlifedata.com/resource/pubmed/commentcorrection/9687486-9016515, http://linkedlifedata.com/resource/pubmed/commentcorrection/9687486-9097448, http://linkedlifedata.com/resource/pubmed/commentcorrection/9687486-9115194, http://linkedlifedata.com/resource/pubmed/commentcorrection/9687486-9254694, http://linkedlifedata.com/resource/pubmed/commentcorrection/9687486-9292985, http://linkedlifedata.com/resource/pubmed/commentcorrection/9687486-9325423, http://linkedlifedata.com/resource/pubmed/commentcorrection/9687486-9336937, http://linkedlifedata.com/resource/pubmed/commentcorrection/9687486-9350723, http://linkedlifedata.com/resource/pubmed/commentcorrection/9687486-9361400, http://linkedlifedata.com/resource/pubmed/commentcorrection/9687486-9399790, http://linkedlifedata.com/resource/pubmed/commentcorrection/9687486-9421915, http://linkedlifedata.com/resource/pubmed/commentcorrection/9687486-9440526, http://linkedlifedata.com/resource/pubmed/commentcorrection/9687486-9546163, http://linkedlifedata.com/resource/pubmed/commentcorrection/9687486-9546186
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Aug
pubmed:issn
0099-2240
pubmed:author
pubmed:issnType
Print
pubmed:volume
64
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
3110-3
pubmed:dateRevised
2009-11-18
pubmed:meshHeading
pubmed:year
1998
pubmed:articleTitle
Specific ribosomal DNA sequences from diverse environmental settings correlate with experimental contaminants.
pubmed:affiliation
Departments of Plant and Microbial Biology and Molecular and Cell Biology, University of California, Berkeley, California 94720-3102, USA.
pubmed:publicationType
Journal Article, Research Support, U.S. Gov't, P.H.S., Research Support, U.S. Gov't, Non-P.H.S.