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rdf:type |
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lifeskim:mentions |
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pubmed:issue |
3
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pubmed:dateCreated |
1998-8-12
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pubmed:abstractText |
This study examined the adhesive interactions of peripheral blood NK cells with P- and E-selectin and analyzed the effect of IL-12 on the binding of NK cells to these selectins. P-selectin glycoprotein ligand-1 (PSGL-1) is expressed on most resting and IL-12-activated NK cells. However, the percentage of resting NK cells bound to P-selectin-IgG was 15%, and that of activated NK cells bound to P-selectin-IgG was 65%. Furthermore, the number of IL-12-activated NK cells bound to P-selectin-transfected Chinese hamster ovary cells was significantly higher than that of resting NK cells under flow conditions. These interactions were abolished by the incubation of these NK cells with anti-PSGL-1 (PL-1) mAb. Thus, PSGL-1/P-selectin interaction is important in the binding of resting and activated NK cells to P-selectin. NK cells express sialyl-Lewis(x) (sLe(x)) structure recognized by anti-sLe(x) mAb (KM-93), and IL-12 activation of NK cells increased the mean fluorescence intensity of KM-93-reactive NK cells. Adhesion of IL-12-activated NK cells to E-selectin-transfected Chinese hamster ovary cells was stronger than that of resting NK cells under flow conditions. These interactions were reduced markedly by incubation with anti-sLe(x) mAb. Thus, sLe(x) is the major ligand of resting and activated NK cells for E-selectin. These findings indicate that IL-12 stimulation of NK cells promotes their adhesion activity to endothelial selectins.
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
AIM
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pubmed:chemical |
http://linkedlifedata.com/resource/pubmed/chemical/5-acetylneuraminyl-(2-3)-galactosyl-...,
http://linkedlifedata.com/resource/pubmed/chemical/Antibodies, Monoclonal,
http://linkedlifedata.com/resource/pubmed/chemical/E-Selectin,
http://linkedlifedata.com/resource/pubmed/chemical/Immunoglobulin G,
http://linkedlifedata.com/resource/pubmed/chemical/Interleukin-12,
http://linkedlifedata.com/resource/pubmed/chemical/Interleukin-2,
http://linkedlifedata.com/resource/pubmed/chemical/L-Selectin,
http://linkedlifedata.com/resource/pubmed/chemical/Lewis Blood-Group System,
http://linkedlifedata.com/resource/pubmed/chemical/Membrane Glycoproteins,
http://linkedlifedata.com/resource/pubmed/chemical/Oligosaccharides,
http://linkedlifedata.com/resource/pubmed/chemical/P-Selectin,
http://linkedlifedata.com/resource/pubmed/chemical/P-selectin ligand protein
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pubmed:status |
MEDLINE
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pubmed:month |
Aug
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pubmed:issn |
0022-1767
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pubmed:author |
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pubmed:issnType |
Print
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pubmed:day |
1
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pubmed:volume |
161
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
1140-5
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pubmed:dateRevised |
2006-11-15
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pubmed:meshHeading |
pubmed-meshheading:9686572-Animals,
pubmed-meshheading:9686572-Antibodies, Monoclonal,
pubmed-meshheading:9686572-CHO Cells,
pubmed-meshheading:9686572-Cell Adhesion,
pubmed-meshheading:9686572-Cricetinae,
pubmed-meshheading:9686572-E-Selectin,
pubmed-meshheading:9686572-Flow Cytometry,
pubmed-meshheading:9686572-Humans,
pubmed-meshheading:9686572-Immunoglobulin G,
pubmed-meshheading:9686572-Interleukin-12,
pubmed-meshheading:9686572-Interleukin-2,
pubmed-meshheading:9686572-Interphase,
pubmed-meshheading:9686572-Killer Cells, Natural,
pubmed-meshheading:9686572-L-Selectin,
pubmed-meshheading:9686572-Lewis Blood-Group System,
pubmed-meshheading:9686572-Lymphocyte Activation,
pubmed-meshheading:9686572-Membrane Glycoproteins,
pubmed-meshheading:9686572-Oligosaccharides,
pubmed-meshheading:9686572-P-Selectin,
pubmed-meshheading:9686572-Rheology,
pubmed-meshheading:9686572-Transfection
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pubmed:year |
1998
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pubmed:articleTitle |
IL-12 promotes the adhesion of NK cells to endothelial selectins under flow conditions.
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pubmed:affiliation |
Department of Otorhinolaryngology, Yokohama City University, School of Medicine, Japan.
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pubmed:publicationType |
Journal Article,
Research Support, Non-U.S. Gov't
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