Switch to
| Predicate | Object |
|---|---|
| rdf:type | |
| lifeskim:mentions | |
| pubmed:issue |
1-2
|
| pubmed:dateCreated |
1998-9-21
|
| pubmed:abstractText |
The intracerebroventricular (i.c.v.) administration of interleukin-1beta (IL-1beta) induces anorexia in rats at doses that yield estimated pathophysiological concentrations in the cerebrospinal fluid. IL-1beta also induces anorexia when administered into the hypothalamic ventromedial nucleus (VMN), an important brain site for the control of feeding. A variety of guanine nucleotide binding protein (G-protein) coupled receptors (e. g., for neurotransmitters and neuropeptides) participate in the integrative regulation of feeding. Our previous studies reported that the VMN G-protein alphaO common subunit subclass is involved in the control of normal feeding, and that IL-1beta modulates calcium channel currents via a pertussis toxin (PTX)-sensitive G-protein (GalphaO/Galphai). Here, we examined the profile of GalphaO protein expression in the hypothalamic VMN during IL-1beta-induced anorexia. Intracerebroventricular microinfusion of IL-1beta (0.5 to 8.0 ng/24 h for 72 h) into the third cerebral ventricle dose-dependently induced anorexia (p<0.001) and decreased the VMN GalphaO common protein levels (p<0.001). Heat-inactivated IL-1beta and IL-1beta plus IL-1 receptor antagonist (a competitive inhibitor of IL-1beta action) had no effect on food intake or on VMN GalphaO common protein content. RT-PCR analysis of VMN RNA from IL-1beta-treated rats generated an expression profile for GalphaO common subunit; however, no modulation at the mRNA level was observed. The results suggest that anorexia induced by the central administration of IL-1beta involves modification of G-protein alphaO common subunit profile in the central nervous system.
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| pubmed:language |
eng
|
| pubmed:journal | |
| pubmed:citationSubset |
IM
|
| pubmed:chemical |
http://linkedlifedata.com/resource/pubmed/chemical/DNA Primers,
http://linkedlifedata.com/resource/pubmed/chemical/GTP-Binding Proteins,
http://linkedlifedata.com/resource/pubmed/chemical/Interleukin-1,
http://linkedlifedata.com/resource/pubmed/chemical/Pertussis Toxin,
http://linkedlifedata.com/resource/pubmed/chemical/RNA, Messenger,
http://linkedlifedata.com/resource/pubmed/chemical/Virulence Factors, Bordetella
|
| pubmed:status |
MEDLINE
|
| pubmed:month |
Jul
|
| pubmed:issn |
0169-328X
|
| pubmed:author | |
| pubmed:copyrightInfo |
Copyright 1998 Elsevier Science B.V. All rights reserved.
|
| pubmed:issnType |
Print
|
| pubmed:day |
15
|
| pubmed:volume |
58
|
| pubmed:owner |
NLM
|
| pubmed:authorsComplete |
Y
|
| pubmed:pagination |
188-94
|
| pubmed:dateRevised |
2006-11-15
|
| pubmed:meshHeading |
pubmed-meshheading:9685638-Animals,
pubmed-meshheading:9685638-Anorexia,
pubmed-meshheading:9685638-Cerebral Ventricles,
pubmed-meshheading:9685638-DNA Primers,
pubmed-meshheading:9685638-Energy Intake,
pubmed-meshheading:9685638-Feeding Behavior,
pubmed-meshheading:9685638-GTP-Binding Proteins,
pubmed-meshheading:9685638-Hypothalamus,
pubmed-meshheading:9685638-Infusions, Parenteral,
pubmed-meshheading:9685638-Interleukin-1,
pubmed-meshheading:9685638-Male,
pubmed-meshheading:9685638-Pertussis Toxin,
pubmed-meshheading:9685638-Polymerase Chain Reaction,
pubmed-meshheading:9685638-RNA, Messenger,
pubmed-meshheading:9685638-Rats,
pubmed-meshheading:9685638-Rats, Wistar,
pubmed-meshheading:9685638-Ventromedial Hypothalamic Nucleus,
pubmed-meshheading:9685638-Virulence Factors, Bordetella
|
| pubmed:year |
1998
|
| pubmed:articleTitle |
In vivo IL-1beta-induced modulation of G-protein alphaO subunit subclass in the hypothalamic ventromedial nucleus: implications to IL-1beta-associated anorexia.
|
| pubmed:affiliation |
Division of Molecular Biology, School of Life and Health Sciences, University of Delaware, Newark, DE 19716-2590, USA. crps@udel.edu
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| pubmed:publicationType |
Journal Article,
Research Support, U.S. Gov't, P.H.S.,
Research Support, Non-U.S. Gov't
|