Linked Life Data

9684761

Source:http://linkedlifedata.com/resource/pubmed/id/9684761

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
2
pubmed:dateCreated
1998-10-9
pubmed:abstractText
Nitric oxide is known to attenuate human platelet activation. Mechanistically this is achieved by stimulation of soluble guanylyl cyclase, followed by cGMP production, and concomitant protein phosphorylation. Although inhibitory actions of nitric oxide on various platelet parameters are well documented, considerably less information is available on the reversibility of this effect. In order to study the onset of proaggregatory signaling pathways after ceasing nitric oxide generation we used the nitric oxide-donor sodium nitroprusside in combination with cyanide. For sodium nitroprusside the generation of nitric oxide requires the release of cyanide prior to nitric oxide. Furthermore, the addition of exogenous cyanide blocks nitric oxide liberation from the nitric oxide-donor. Our data indicate, that the inhibitory potency of sodium nitroprusside on platelet aggregation, calcium mobilization, and a cGMP increase is reversed by cyanide addition. We put special attention to nitric oxide-mediated cGMP increase, followed by the extreme rapid cGMP degradation after cyanide administration followed by the onset of major proaggregatory signaling pathways. Our study aims at the physiological importance of a permanently active, probably shear stress induced nitric oxide-synthase in endothelial cells that functions as a negative thromboregulatory mechanism.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Apr
pubmed:issn
0049-3848
pubmed:author
pubmed:issnType
Print
pubmed:day
15
pubmed:volume
90
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
83-91
pubmed:dateRevised
2006-11-15
pubmed:meshHeading
pubmed:year
1998
pubmed:articleTitle
Rapid reversibility of nitric oxide induced platelet inhibition.
pubmed:affiliation
University of Erlangen-Nürnberg, Faculty of Medicine, Erlangen, Germany. mfm423@rzmail.uni-erlangen.de
pubmed:publicationType
Journal Article, In Vitro, Research Support, Non-U.S. Gov't