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| Predicate | Object |
|---|---|
| rdf:type | |
| lifeskim:mentions | |
| pubmed:issue |
5
|
| pubmed:dateCreated |
1998-8-13
|
| pubmed:abstractText |
The development of a disposable amperometric immunomigration sensor for the detection of triazine pesticides in real samples is presented. Thick film electrodes printed on PVC were used as strip-type transducers. Monoclonal antibodies against atrazine and terbutylazine served as biorecognition element. For the generation and amplification of the signal, hapten-tagged liposomes entrapping ascorbic acid as a marker molecule were chosen. A capillary gap and a wicking filter membrane strip served as migration zone. For signal detection on a graphite electrode, liposomes were lysed by Triton X-100 and the released ascorbic acid was quantified at a potential of + 300 mV vs printed Ag/AgCl. Signal response time was 1-3 min, and sensitivity of measurements in tap water was below 1 microgram l-1 of atrazine and terbutylazine. Atrazine determinations in soil extracts correlated well with standard procedures based on ELISA and HPLC.
|
| pubmed:language |
eng
|
| pubmed:journal | |
| pubmed:citationSubset |
IM
|
| pubmed:chemical | |
| pubmed:status |
MEDLINE
|
| pubmed:month |
Mar
|
| pubmed:issn |
0956-5663
|
| pubmed:author | |
| pubmed:issnType |
Print
|
| pubmed:day |
15
|
| pubmed:volume |
13
|
| pubmed:owner |
NLM
|
| pubmed:authorsComplete |
Y
|
| pubmed:pagination |
519-29
|
| pubmed:dateRevised |
2009-7-14
|
| pubmed:meshHeading | |
| pubmed:year |
1998
|
| pubmed:articleTitle |
Development of a new immunosensor for pesticide detection: a disposable system with liposome-enhancement and amperometric detection.
|
| pubmed:affiliation |
Institute for Technical Biochemistry, University of Stuttgart, Germany.
|
| pubmed:publicationType |
Journal Article,
Research Support, Non-U.S. Gov't
|