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rdf:type |
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lifeskim:mentions |
umls-concept:C0017337,
umls-concept:C0033684,
umls-concept:C0205117,
umls-concept:C0205314,
umls-concept:C0207324,
umls-concept:C0392760,
umls-concept:C0596988,
umls-concept:C0679622,
umls-concept:C0752063,
umls-concept:C1314939,
umls-concept:C1947912,
umls-concept:C1960782,
umls-concept:C2700640
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pubmed:issue |
1
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pubmed:dateCreated |
1998-9-18
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pubmed:databankReference |
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pubmed:abstractText |
The Hansenula polymorpha per6-210 mutant is impaired in respect of growth on methanol (Mut-) and is characterized by aberrant peroxisome formation. The functionally complementing DNA fragment contains two open reading frames. The first encodes dihydroxyacetone kinase (DAK), a cytosolic enzyme essential for formaldehyde assimilation; the second ORF codes for a novel protein (Pak1p). We have demonstrated that per6-210 cells lack DAK activity, causing the Mut- phenotype, and have strongly reduced levels of Pak1p, resulting in peroxisomal defects. Sequence analysis revealed that per6-210 contains a mutation in the 3' end of the DAK coding region, which overlaps with the promoter region of PAK1. Possibly this mutation also negatively affects PAK1 expression.
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
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pubmed:status |
MEDLINE
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pubmed:month |
Jul
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pubmed:issn |
0172-8083
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pubmed:author |
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pubmed:issnType |
Print
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pubmed:volume |
34
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
1-11
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pubmed:dateRevised |
2008-11-21
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pubmed:meshHeading |
pubmed-meshheading:9683670-Amino Acid Sequence,
pubmed-meshheading:9683670-Cloning, Molecular,
pubmed-meshheading:9683670-DNA, Complementary,
pubmed-meshheading:9683670-Fungal Proteins,
pubmed-meshheading:9683670-Magnetic Resonance Spectroscopy,
pubmed-meshheading:9683670-Methanol,
pubmed-meshheading:9683670-Microbodies,
pubmed-meshheading:9683670-Microscopy, Electron,
pubmed-meshheading:9683670-Molecular Sequence Data,
pubmed-meshheading:9683670-Mutation,
pubmed-meshheading:9683670-Neurospora crassa,
pubmed-meshheading:9683670-Open Reading Frames,
pubmed-meshheading:9683670-Phosphotransferases (Alcohol Group Acceptor),
pubmed-meshheading:9683670-Pichia,
pubmed-meshheading:9683670-Promoter Regions, Genetic,
pubmed-meshheading:9683670-Restriction Mapping,
pubmed-meshheading:9683670-Sequence Analysis,
pubmed-meshheading:9683670-Sequence Homology
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pubmed:year |
1998
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pubmed:articleTitle |
The Hansenula polymorpha per6 mutant is affected in two adjacent genes which encode dihydroxyacetone kinase and a novel protein, Pak1p, involved in peroxisome integrity.
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pubmed:affiliation |
Eukaryotic Microbiology, Groningen Biomolecular Sciences and Biotechnology Institute, University of Groningen, Kerklaan 30, NL-9751 NN Haren, The Netherlands. IJVDKLEI@BIOL.RUG.NL
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pubmed:publicationType |
Journal Article,
Comparative Study
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