Linked Life Data

9683643

Source:http://linkedlifedata.com/resource/pubmed/id/9683643

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
2
pubmed:dateCreated
1998-9-11
pubmed:abstractText
Sexual development in Aspergillus nidulans is a morphogenetic differentiation process triggered by internal and environmental signals. As a first step in analyzing the developmental pathway at the molecular level, laccase II (EC 1.10.3.2), which is specifically expressed in early stages of fruitbodies, was isolated. The enzyme was purified to apparent homogeneity from a mutant strain (SMS1) in which the sexual cycle dominates and the number of cleistothecia is increased tenfold. Laccase II was enriched 560-fold to a specific activity of 892 U (mg protein)-1. The apparent molecular mass was determined to be 80 kDa under denaturing conditions and to be 100-120 kDa under native conditions. The internal peptide sequences gained from the protein will allow the isolation of the corresponding gene as a first step in determining the key regulators of sexual development.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Aug
pubmed:issn
0302-8933
pubmed:author
pubmed:issnType
Print
pubmed:volume
170
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
78-84
pubmed:dateRevised
2006-11-15
pubmed:meshHeading
pubmed:year
1998
pubmed:articleTitle
Purification and characterization of laccase II of Aspergillus nidulans.
pubmed:affiliation
Laboratorium für Mikrobiologie, Philipps-Universität Marburg and Max-Planck-Institut für terrestrische Mikrobiologie, Karl-von-Frisch-Strasse, D-35 043 Marburg, Germany.
pubmed:publicationType
Journal Article, Research Support, Non-U.S. Gov't