Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
2
pubmed:dateCreated
1998-8-21
pubmed:databankReference
pubmed:abstractText
A novel 44-kDa gene product (D123) has been proposed as necessary for S-phase entry of the cell cycle: a point mutation resulted in a temperature-sensitive arrest in G1-phase. From human fibrosarcoma cDNA library, we have isolated an identical gene and studied its sequence and mRNA and protein expression. Compared with D123, three nucleotide differences within the human coding sequence, plus others, result in a change of two amino acids. A partial sequence similarity has been found with a yeast gene of unknown function. The protein has several potential phosphorylation sites, is highly hydrophilic, and may be highly structured in alpha-helix. The mRNA is abundantly expressed by a variety of normal and transformed cells and by all tissues examined, being most highly expressed in testis. Specific antibodies, raised against a rhD123 polypeptide, recognize a major 42- to 44-kDa molecule in crude extract of various human cell lines. Immunohistochemistry reveals that D123 protein is not homogeneously expressed: it is detected, often in granular vescicles, in the cytoplasm of some epithelial, stromal, and sperm cells and in varicosities lining nervous fibers, while it appears to be absent in nuclei, endothelial, and smooth muscle cells. The precise link between cytoplasmic occurrence of D123 and cell cycle progression still remains to be clarified.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Aug
pubmed:issn
0014-4827
pubmed:author
pubmed:copyrightInfo
Copyright 1998 Academic Press.
pubmed:issnType
Print
pubmed:day
1
pubmed:volume
242
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
451-9
pubmed:dateRevised
2007-11-15
pubmed:meshHeading
pubmed-meshheading:9683532-Amino Acid Sequence, pubmed-meshheading:9683532-Amino Acids, pubmed-meshheading:9683532-Animals, pubmed-meshheading:9683532-Base Sequence, pubmed-meshheading:9683532-Blotting, Northern, pubmed-meshheading:9683532-Blotting, Western, pubmed-meshheading:9683532-Cell Cycle Proteins, pubmed-meshheading:9683532-Cell Line, pubmed-meshheading:9683532-Cell Line, Transformed, pubmed-meshheading:9683532-DNA, Complementary, pubmed-meshheading:9683532-Gene Expression, pubmed-meshheading:9683532-Humans, pubmed-meshheading:9683532-Immunohistochemistry, pubmed-meshheading:9683532-Male, pubmed-meshheading:9683532-Molecular Sequence Data, pubmed-meshheading:9683532-Protein Processing, Post-Translational, pubmed-meshheading:9683532-Proteins, pubmed-meshheading:9683532-RNA, Messenger, pubmed-meshheading:9683532-Rabbits, pubmed-meshheading:9683532-Rats, pubmed-meshheading:9683532-Sequence Alignment, pubmed-meshheading:9683532-Sequence Analysis, DNA, pubmed-meshheading:9683532-Statistics as Topic, pubmed-meshheading:9683532-Testis, pubmed-meshheading:9683532-Tissue Distribution, pubmed-meshheading:9683532-Tumor Cells, Cultured
pubmed:year
1998
pubmed:articleTitle
Expression study on D123 gene product: evidence for high positivity in testis.
pubmed:affiliation
Medical School, Department of Biological Chemistry, University of Padua, viale G. Colombo 3, Padua, 35121, Italy. onisto@civ.bio.unipd.it
pubmed:publicationType
Journal Article, Research Support, Non-U.S. Gov't