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rdf:type |
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lifeskim:mentions |
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pubmed:issue |
6
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pubmed:dateCreated |
1998-8-13
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pubmed:abstractText |
Thrombocytopenia is a frequent feature of myelodysplastic syndromes (MDS) that could be improved by the use of recombinant human megakaryocyte growth and development factor (rHuMGDF). Using short-term liquid cultures and progenitor assays, we have found that rHuMGDF stimulated DNA synthesis and potentiated leukemic cluster growth of bone marrow mononuclear cells in 10/38 MDS cases (26%). Cytogenetically malignant colonies were detectable in rHuMGDF-stimulated cultures (n=3) by fluorescence in situ hybridization. rHuMGDF was able to stimulate CFU-MK formation in 45% of the samples tested. Finally, rHuMGDF-induced blast cell proliferation correlated with elevated expression of c-MPL, previously identified as a bad prognosis factor in MDS.
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
http://linkedlifedata.com/resource/pubmed/chemical/DNA,
http://linkedlifedata.com/resource/pubmed/chemical/MPL protein, human,
http://linkedlifedata.com/resource/pubmed/chemical/Methylcellulose,
http://linkedlifedata.com/resource/pubmed/chemical/Neoplasm Proteins,
http://linkedlifedata.com/resource/pubmed/chemical/Proto-Oncogene Proteins,
http://linkedlifedata.com/resource/pubmed/chemical/RNA,
http://linkedlifedata.com/resource/pubmed/chemical/Receptors, Cytokine,
http://linkedlifedata.com/resource/pubmed/chemical/Receptors, Immunologic,
http://linkedlifedata.com/resource/pubmed/chemical/Receptors, Thrombopoietin,
http://linkedlifedata.com/resource/pubmed/chemical/Recombinant Proteins,
http://linkedlifedata.com/resource/pubmed/chemical/Thrombopoietin
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pubmed:status |
MEDLINE
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pubmed:month |
Jun
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pubmed:issn |
0145-2126
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pubmed:author |
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pubmed:issnType |
Print
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pubmed:volume |
22
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
527-35
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pubmed:dateRevised |
2006-11-15
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pubmed:meshHeading |
pubmed-meshheading:9678719-Blast Crisis,
pubmed-meshheading:9678719-Blotting, Northern,
pubmed-meshheading:9678719-Cell Culture Techniques,
pubmed-meshheading:9678719-Cell Division,
pubmed-meshheading:9678719-Clone Cells,
pubmed-meshheading:9678719-Colony-Forming Units Assay,
pubmed-meshheading:9678719-DNA,
pubmed-meshheading:9678719-Hematopoietic Stem Cells,
pubmed-meshheading:9678719-Humans,
pubmed-meshheading:9678719-In Situ Hybridization, Fluorescence,
pubmed-meshheading:9678719-Karyotyping,
pubmed-meshheading:9678719-Megakaryocytes,
pubmed-meshheading:9678719-Methylcellulose,
pubmed-meshheading:9678719-Myelodysplastic Syndromes,
pubmed-meshheading:9678719-Neoplasm Proteins,
pubmed-meshheading:9678719-Proto-Oncogene Proteins,
pubmed-meshheading:9678719-RNA,
pubmed-meshheading:9678719-Receptors, Cytokine,
pubmed-meshheading:9678719-Receptors, Immunologic,
pubmed-meshheading:9678719-Receptors, Thrombopoietin,
pubmed-meshheading:9678719-Recombinant Proteins,
pubmed-meshheading:9678719-Thrombopoietin
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pubmed:year |
1998
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pubmed:articleTitle |
Analysis of megakaryocyte growth and development factor (thrombopoietin) effects on blast cell and megakaryocyte growth in myelodysplasia.
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pubmed:affiliation |
Laboratoire d'Hématologie et INSERM U363 ICGM, Université Paris V, Hôpital Cochin, France.
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pubmed:publicationType |
Journal Article
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