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| Predicate | Object |
|---|---|
| rdf:type | |
| lifeskim:mentions | |
| pubmed:issue |
31
|
| pubmed:dateCreated |
1998-9-10
|
| pubmed:databankReference | |
| pubmed:abstractText |
This study demonstrates that the transcriptional repressor sequence of the rat vasoactive intestinal peptide receptor (VIPR) gene constitutes a 42-base pair core element that is the binding site for a nuclear protein. We showed that this element was able to confer transcriptional repression to a heterologous promoter and that deletion or point mutations within this element resulted in loss of transcriptional repression. Southwestern blot analysis indicated that the VIPR repressor element interacts specifically with a nuclear protein of about 72 kDa. By screening a rat lung expression library coupled with rapid amplification of cDNA ends polymerase chain reactions, we isolated a cDNA clone (designated as VIPR-RP) that contains an open reading frame of 656 amino acids. VIPR-RP is 78% identical to a previously characterized protein, differentiation-specific element-binding protein, which is a member of a family of proteins including components of the DNA replication factor C complex. However, VIPR-RP cDNA encodes for a much smaller protein than differentiation-specific element-binding protein because of a frameshift. VIPR-RP mRNA is expressed in multiple tissues, including lung, liver, brain, heart, kidney, spleen, and testis. VIPR-RP protein specifically interacts with the VIPR repressor element as demonstrated by gel shift assays. Transfection of VIP-RP expression vector into Cos cells resulted in transcriptional repression of a reporter construct containing multiple copies of the VIPR repressor element. These results indicate that VIPR-RP is a novel transcriptional repressor protein that regulates VIPR expression.
|
| pubmed:grant | |
| pubmed:language |
eng
|
| pubmed:journal | |
| pubmed:citationSubset |
IM
|
| pubmed:chemical |
http://linkedlifedata.com/resource/pubmed/chemical/DNA-Binding Proteins,
http://linkedlifedata.com/resource/pubmed/chemical/Nuclear Proteins,
http://linkedlifedata.com/resource/pubmed/chemical/RNA, Messenger,
http://linkedlifedata.com/resource/pubmed/chemical/Receptors, Vasoactive Intestinal...,
http://linkedlifedata.com/resource/pubmed/chemical/Replication Protein C,
http://linkedlifedata.com/resource/pubmed/chemical/Repressor Proteins
|
| pubmed:status |
MEDLINE
|
| pubmed:month |
Jul
|
| pubmed:issn |
0021-9258
|
| pubmed:author | |
| pubmed:issnType |
Print
|
| pubmed:day |
31
|
| pubmed:volume |
273
|
| pubmed:owner |
NLM
|
| pubmed:authorsComplete |
Y
|
| pubmed:pagination |
19902-8
|
| pubmed:dateRevised |
2008-11-21
|
| pubmed:meshHeading |
pubmed-meshheading:9677428-Amino Acid Sequence,
pubmed-meshheading:9677428-Animals,
pubmed-meshheading:9677428-Base Sequence,
pubmed-meshheading:9677428-COS Cells,
pubmed-meshheading:9677428-Cloning, Molecular,
pubmed-meshheading:9677428-DNA-Binding Proteins,
pubmed-meshheading:9677428-Gene Expression Regulation,
pubmed-meshheading:9677428-Genes, Reporter,
pubmed-meshheading:9677428-Molecular Sequence Data,
pubmed-meshheading:9677428-Mutation,
pubmed-meshheading:9677428-Nuclear Proteins,
pubmed-meshheading:9677428-Promoter Regions, Genetic,
pubmed-meshheading:9677428-RNA, Messenger,
pubmed-meshheading:9677428-Rats,
pubmed-meshheading:9677428-Receptors, Vasoactive Intestinal Peptide,
pubmed-meshheading:9677428-Replication Protein C,
pubmed-meshheading:9677428-Repressor Proteins,
pubmed-meshheading:9677428-Sequence Alignment,
pubmed-meshheading:9677428-Sequence Analysis, DNA,
pubmed-meshheading:9677428-Transfection
|
| pubmed:year |
1998
|
| pubmed:articleTitle |
Molecular cloning of a novel transcriptional repressor protein of the rat type 1 vasoactive intestinal peptide receptor gene.
|
| pubmed:affiliation |
Division of Endocrinology, Cedars-Sinai Research Institute, UCLA School of Medicine, Los Angeles, California 90048, USA.
|
| pubmed:publicationType |
Journal Article,
Research Support, U.S. Gov't, P.H.S.,
Research Support, Non-U.S. Gov't
|