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rdf:type |
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lifeskim:mentions |
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pubmed:dateCreated |
1998-9-23
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pubmed:abstractText |
We have investigated the cell cycle inhibition mechanism and primary target of tryprostatin A (TPS-A) purified from Aspergillus fumigatus. TPS-A inhibited cell cycle progression of asynchronously cultured 3Y1 cells in the M phase in a dose- and time-dependent manner. In contrast, TPS-B (the demethoxy analogue of TPS-A) showed cell-cycle non-specific inhibition on cell growth even though it inhibited cell growth at lower concentrations than TPS-A. TPS-A treatment induced the reversible disruption of the cytoplasmic microtubules of 3Y1 cells as observed by indirect immunofluorescence microscopy in the range of concentrations that specifically inhibited M-phase progression. TPS-A inhibited the assembly in vitro of microtubules purified from bovine brains (40% inhibition at 250 microM); however, there was little or no effect on the self-assembly of purified tubulin when polymerization was induced by glutamate even at 250 microM TPS-A. TPS-A did not inhibit assembly promoted by taxol or by digestion of the C-terminal domain of tubulin. However, TPS-A blocked the tubulin assembly induced by inducers interacting with the C-terminal domain, microtubule-associated protein 2 (MAP2), tau and poly-(l-lysine). These results indicate that TPS-A is a novel inhibitor of MAP-dependent microtubule assembly and, through the disruption of the microtubule spindle, specifically inhibits cell cycle progression at the M phase.
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pubmed:commentsCorrections |
http://linkedlifedata.com/resource/pubmed/commentcorrection/9677311-1057175,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9677311-1353706,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9677311-1383436,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9677311-1599956,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9677311-1633159,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9677311-166944,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9677311-1818332,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9677311-1874739,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9677311-1984408,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9677311-2208288,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9677311-2428648,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9677311-3115177,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9677311-3120782,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9677311-3122323,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9677311-3142041,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9677311-3349462,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9677311-3606749,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9677311-3894367,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9677311-3965457,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9677311-423966,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9677311-4514990,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9677311-6385005,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9677311-6487596,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9677311-6548750,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9677311-6833250,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9677311-729584,
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http://linkedlifedata.com/resource/pubmed/commentcorrection/9677311-758003,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9677311-811253,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9677311-8402630,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9677311-852577,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9677311-8557590,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9677311-8647876,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9677311-8698634,
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http://linkedlifedata.com/resource/pubmed/commentcorrection/9677311-8813133,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9677311-9020786,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9677311-9043671,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9677311-9119003,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9677311-9190213,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9677311-954003,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9677311-985482
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
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pubmed:status |
MEDLINE
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pubmed:month |
Aug
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pubmed:issn |
0264-6021
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pubmed:author |
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pubmed:issnType |
Print
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pubmed:day |
1
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pubmed:volume |
333 ( Pt 3)
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
543-8
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pubmed:dateRevised |
2009-11-18
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pubmed:meshHeading |
pubmed-meshheading:9677311-Animals,
pubmed-meshheading:9677311-Cattle,
pubmed-meshheading:9677311-Cell Cycle,
pubmed-meshheading:9677311-Cells, Cultured,
pubmed-meshheading:9677311-Fibroblasts,
pubmed-meshheading:9677311-Indole Alkaloids,
pubmed-meshheading:9677311-Indoles,
pubmed-meshheading:9677311-Microtubule-Associated Proteins,
pubmed-meshheading:9677311-Microtubules,
pubmed-meshheading:9677311-Piperazines,
pubmed-meshheading:9677311-Rats,
pubmed-meshheading:9677311-Tubulin,
pubmed-meshheading:9677311-tau Proteins
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pubmed:year |
1998
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pubmed:articleTitle |
Tryprostatin A, a specific and novel inhibitor of microtubule assembly.
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pubmed:affiliation |
Antibiotics Laboratory, The Institute of Physical and Chemical Research (RIKEN), Hirosawa 2-1, Wako, Saitama 351-01, Japan.
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pubmed:publicationType |
Journal Article,
Research Support, Non-U.S. Gov't
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