|
rdf:type |
|
|
lifeskim:mentions |
|
|
pubmed:issue |
2
|
|
pubmed:dateCreated |
1998-8-19
|
|
pubmed:databankReference |
|
|
pubmed:abstractText |
The previously isolated non-phosphinothricin tripeptide producing Streptomyces viridochromogenes gene disruption mutant SP62/2 was used to identify and analyze genes encoding early steps of the phosphinothricin tripeptide biosynthesis. Cross-feeding and bioconversion experiments between SP62/2 and known non-phosphinothricin tripeptide producing mutants or presumptive phosphinothricin tripeptide precursors revealed that SP62/2 was blocked in step one or two of the phosphinothricin tripeptide biosynthesis. It was shown that the block in the biosynthesis is due to the integration of a temperature-sensitive plasmid by illegitimate recombination into the phosphinothricin tripeptide biosynthetic gene cluster. The corresponding region was isolated from the wild-type. A 2.7-kb DNA fragment was analyzed comprising three ORFs (ppm, ppd, orfX) which are probably translationally coupled. The ppm gene encodes a protein which is similar to PEP-phosphomutases and the deduced Ppd product shows similarity to the phosphonopyruvate decarboxylase from Streptomyces wedmorensis.
|
|
pubmed:language |
eng
|
|
pubmed:journal |
|
|
pubmed:citationSubset |
IM
|
|
pubmed:chemical |
|
|
pubmed:status |
MEDLINE
|
|
pubmed:month |
Jun
|
|
pubmed:issn |
0378-1097
|
|
pubmed:author |
|
|
pubmed:issnType |
Print
|
|
pubmed:day |
15
|
|
pubmed:volume |
163
|
|
pubmed:owner |
NLM
|
|
pubmed:authorsComplete |
Y
|
|
pubmed:pagination |
149-57
|
|
pubmed:dateRevised |
2006-11-15
|
|
pubmed:meshHeading |
pubmed-meshheading:9673017-Amino Acid Sequence,
pubmed-meshheading:9673017-Aminobutyric Acids,
pubmed-meshheading:9673017-Anti-Bacterial Agents,
pubmed-meshheading:9673017-Bacterial Proteins,
pubmed-meshheading:9673017-Bacteriophage lambda,
pubmed-meshheading:9673017-Base Sequence,
pubmed-meshheading:9673017-Blotting, Southern,
pubmed-meshheading:9673017-Carboxy-Lyases,
pubmed-meshheading:9673017-Gene Library,
pubmed-meshheading:9673017-Molecular Sequence Data,
pubmed-meshheading:9673017-Mutation,
pubmed-meshheading:9673017-Peptide Synthases,
pubmed-meshheading:9673017-Peptides,
pubmed-meshheading:9673017-Phosphotransferases (Phosphomutases),
pubmed-meshheading:9673017-Plasmids,
pubmed-meshheading:9673017-Sequence Analysis, DNA,
pubmed-meshheading:9673017-Streptomyces
|
|
pubmed:year |
1998
|
|
pubmed:articleTitle |
Isolation and characterization of the PEP-phosphomutase and the phosphonopyruvate decarboxylase genes from the phosphinothricin tripeptide producer Streptomyces viridochromogenes Tü494.
|
|
pubmed:affiliation |
Eberhard-Karls-Universität Tübingen, Germany.
|
|
pubmed:publicationType |
Journal Article
|
