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rdf:type |
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lifeskim:mentions |
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pubmed:issue |
4
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pubmed:dateCreated |
1978-8-14
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pubmed:abstractText |
An inverse relationship between binding of [(14)C]penicillin to cells of Neisseria gonorrhoeae and the minimal inhibitory concentration of penicillin was recently reported by us. Herein we report a similar relationship in binding by cytoplasmic membranes derived from 10 strains of gonococci. Membranes were extracted after Braun homogenization and differential centrifugation of the resulting extracts. Membranes derived from penicillin-susceptible cells bound 140 to 200 nmol of [(14)C]penicillin per g of enzyme, whereas relatively nonsusceptible strains bound 18 to 59 nmol. Binding by cell walls was only a small fraction of the total binding and probably represents contamination of the walls by membrane. Pretreatment of cell membranes with either unlabeled penicillin or cephalothin prior to [(14)C]penicillin exposure markedly decreased (14)C binding. Slab gel electrophoresis of membranes revealed 13 protein bands, 8 of which were labeled.
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pubmed:commentsCorrections |
http://linkedlifedata.com/resource/pubmed/commentcorrection/96727-13130795,
http://linkedlifedata.com/resource/pubmed/commentcorrection/96727-13163342,
http://linkedlifedata.com/resource/pubmed/commentcorrection/96727-14354138,
http://linkedlifedata.com/resource/pubmed/commentcorrection/96727-14907713,
http://linkedlifedata.com/resource/pubmed/commentcorrection/96727-16748652,
http://linkedlifedata.com/resource/pubmed/commentcorrection/96727-409343,
http://linkedlifedata.com/resource/pubmed/commentcorrection/96727-4200865,
http://linkedlifedata.com/resource/pubmed/commentcorrection/96727-4248524,
http://linkedlifedata.com/resource/pubmed/commentcorrection/96727-4330943,
http://linkedlifedata.com/resource/pubmed/commentcorrection/96727-4352815,
http://linkedlifedata.com/resource/pubmed/commentcorrection/96727-4626716,
http://linkedlifedata.com/resource/pubmed/commentcorrection/96727-4630162,
http://linkedlifedata.com/resource/pubmed/commentcorrection/96727-4760132,
http://linkedlifedata.com/resource/pubmed/commentcorrection/96727-4990947,
http://linkedlifedata.com/resource/pubmed/commentcorrection/96727-6033778,
http://linkedlifedata.com/resource/pubmed/commentcorrection/96727-60518,
http://linkedlifedata.com/resource/pubmed/commentcorrection/96727-60519,
http://linkedlifedata.com/resource/pubmed/commentcorrection/96727-63850,
http://linkedlifedata.com/resource/pubmed/commentcorrection/96727-808158
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
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pubmed:status |
MEDLINE
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pubmed:month |
Apr
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pubmed:issn |
0066-4804
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pubmed:author |
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pubmed:issnType |
Print
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pubmed:volume |
13
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
589-97
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pubmed:dateRevised |
2009-11-18
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pubmed:meshHeading |
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pubmed:year |
1978
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pubmed:articleTitle |
Differential binding of penicillin by membrane fractions from penicillin-susceptible and -resistant gonococci.
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pubmed:publicationType |
Journal Article,
Research Support, U.S. Gov't, P.H.S.
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