Linked Life Data

9672604

Source:http://linkedlifedata.com/resource/pubmed/id/9672604

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
12
pubmed:dateCreated
1998-8-27
pubmed:abstractText
Fowlpox virus (FPV) insertion plasmids were constructed that, upon integration into the viral genome via in-vivo recombination, inactivate the viral thymidine kinase (tk) gene. Using this approach, no wild-type virus-free stocks of recombinant virus could be obtained. In contrast, either integration of foreign genes into the intergenic region of the intact FPV tk gene and the open reading frame located downstream, or the functional substitution of the inactivated FPV tk gene by an intact vaccinia virus tk gene resulted in the predicted stable recombinants that were free of wild-type virus. Our results suggest that in already highly attenuated poxvirus strains an intact tk gene is essential for efficient growth of the virus in cell culture.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:issn
0304-8608
pubmed:author
pubmed:issnType
Print
pubmed:volume
142
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
2421-31
pubmed:dateRevised
2006-5-1
pubmed:meshHeading
pubmed:year
1997
pubmed:articleTitle
Role of the fowlpox virus thymidine kinase gene for the growth of FPV recombinants in cell culture.
pubmed:affiliation
Immuno AG, Biomedical Research Center, Orth/Donau, Austria.
pubmed:publicationType
Journal Article