Linked Life Data

9668119

Source:http://linkedlifedata.com/resource/pubmed/id/9668119

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
30
pubmed:dateCreated
1998-8-20
pubmed:abstractText
Kinase domain receptor (KDR) is a high affinity, endothelial cell-specific, autophosphorylating tyrosine kinase receptor for vascular endothelial growth factor. This transcriptionally regulated receptor is a critical mediator of endothelial cell (EC) growth and vascular development. In this study, we identify a DNA element modulating KDR promoter activity and evaluate the nuclear binding proteins accounting for a portion of the cell-type specificity of the region. KDR promoter luciferase activity was retained within -85/+296 and was 10-30-fold higher in EC than non-EC. Electrophoretic mobility shift assays demonstrated specific nuclear protein binding to -85/-64, and single point mutations suggested important binding nucleotides between -79/-68 with five critical bases between -74/-70 (5'-CTCCT-3'). DNA-protein complexes were displaced by Sp1 consensus sequence oligodeoxynucleotides and supershifted by Sp1- and Sp3-specific antibodies. Sp1 and Sp3 protein in EC nuclear extracts bound the -79/-68 region even when all surrounding classic Sp1 recognition sites were removed. Sp1 protein in nuclear extracts was 4-24-fold higher in EC than non-EC, whereas Sp3 was 3-7-fold higher. Sp1/Sp3 ratios in EC were 2-10-fold higher. Overexpression of Sp1 protein increased KDR promoter activity 3-fold in both EC and non-EC, whereas simultaneous co-expression of Sp3 attenuated this response. An Sp1 consensus sequence cis element "decoy" reduced EC KDR promoter activity and mRNA expression by 85 and 69%, respectively. An antisense phosphorothioate oligodeoxynucleotide to Sp1 inhibited Sp1 and KDR protein expression by 66 and 68%, respectively, without changing Sp3 protein expression. These data illustrate that Sp1 and Sp3 modulate KDR promoter activity through a novel recognition binding sequence. However, since Sp1-mediated promoter activation is attenuated by Sp3, endothelial selective KDR promoter activity may be partially regulated by variations in the Sp1/Sp3 ratio.
pubmed:grant
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Jul
pubmed:issn
0021-9258
pubmed:author
pubmed:issnType
Print
pubmed:day
24
pubmed:volume
273
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
19294-303
pubmed:dateRevised
2009-11-19
pubmed:meshHeading
pubmed-meshheading:9668119-Base Sequence, pubmed-meshheading:9668119-Cells, Cultured, pubmed-meshheading:9668119-DNA-Binding Proteins, pubmed-meshheading:9668119-Gene Expression Regulation, pubmed-meshheading:9668119-Humans, pubmed-meshheading:9668119-Molecular Sequence Data, pubmed-meshheading:9668119-Oligonucleotides, Antisense, pubmed-meshheading:9668119-Promoter Regions, Genetic, pubmed-meshheading:9668119-Receptor Protein-Tyrosine Kinases, pubmed-meshheading:9668119-Receptors, Growth Factor, pubmed-meshheading:9668119-Receptors, Vascular Endothelial Growth Factor, pubmed-meshheading:9668119-Sp1 Transcription Factor, pubmed-meshheading:9668119-Sp3 Transcription Factor, pubmed-meshheading:9668119-Thionucleotides, pubmed-meshheading:9668119-Transcription Factors, pubmed-meshheading:9668119-Zinc Fingers
pubmed:year
1998
pubmed:articleTitle
Transcription factors Sp1 and Sp3 alter vascular endothelial growth factor receptor expression through a novel recognition sequence.
pubmed:affiliation
Research Division, Joslin Diabetes Center, Boston, Massachusetts 02215, USA.
pubmed:publicationType
Journal Article, Research Support, U.S. Gov't, P.H.S., Research Support, Non-U.S. Gov't