Linked Life Data

9668038

Source:http://linkedlifedata.com/resource/pubmed/id/9668038

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
30
pubmed:dateCreated
1998-8-20
pubmed:abstractText
Plasma membrane Ca2+-ATPase isoform 2 (PMCA2) exhibits a highly restricted tissue distribution, suggesting that it serves more specialized physiological functions than some of the other isoforms. A unique role in hearing is indicated by the high levels of PMCA2 expression in cochlear outer hair cells and spiral ganglion cells. To analyze the physiological role of PMCA2 we used gene targeting to produce PMCA2-deficient mice. Breeding of heterozygous mice yielded live homozygous mutant offspring. PMCA2-null mice grow more slowly than heterozygous and wild-type mice and exhibit an unsteady gait and difficulties in maintaining balance. Histological analysis of the cerebellum and inner ear of mutant and wild-type mice revealed that null mutants had slightly increased numbers of Purkinje neurons (in which PMCA2 is highly expressed), a decreased thickness of the molecular layer, an absence of otoconia in the vestibular system, and a range of abnormalities of the organ of Corti. Analysis of auditory evoked brainstem responses revealed that homozygous mutants were deaf and that heterozygous mice had a significant hearing loss. These data demonstrate that PMCA2 is required for both balance and hearing and suggest that it may be a major source of the calcium used in the formation and maintenance of otoconia.
pubmed:grant
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Jul
pubmed:issn
0021-9258
pubmed:author
pubmed:issnType
Print
pubmed:day
24
pubmed:volume
273
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
18693-6
pubmed:dateRevised
2011-8-19
pubmed:meshHeading
pubmed-meshheading:9668038-Animals, pubmed-meshheading:9668038-Calcium, pubmed-meshheading:9668038-Calcium-Transporting ATPases, pubmed-meshheading:9668038-Cation Transport Proteins, pubmed-meshheading:9668038-Cell Membrane, pubmed-meshheading:9668038-Deafness, pubmed-meshheading:9668038-Evoked Potentials, Auditory, Brain Stem, pubmed-meshheading:9668038-Gene Targeting, pubmed-meshheading:9668038-Hair Cells, Vestibular, pubmed-meshheading:9668038-In Situ Hybridization, pubmed-meshheading:9668038-Mice, pubmed-meshheading:9668038-Mice, Knockout, pubmed-meshheading:9668038-Otolithic Membrane, pubmed-meshheading:9668038-Plasma Membrane Calcium-Transporting ATPases, pubmed-meshheading:9668038-Postural Balance, pubmed-meshheading:9668038-RNA, Messenger, pubmed-meshheading:9668038-Sensation Disorders
pubmed:year
1998
pubmed:articleTitle
Balance and hearing deficits in mice with a null mutation in the gene encoding plasma membrane Ca2+-ATPase isoform 2.
pubmed:affiliation
Department of Molecular Genetics, Biochemistry and Microbiology, University of Cincinnati College of Medicine, Cincinnati, Ohio 45267, USA.
pubmed:publicationType
Journal Article, Research Support, U.S. Gov't, P.H.S., Research Support, Non-U.S. Gov't