Linked Life Data

9666481

Source:http://linkedlifedata.com/resource/pubmed/id/9666481

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
6
pubmed:dateCreated
1998-9-21
pubmed:abstractText
Using the seafood contaminant domoic acid (an AMPA/kainate receptor agonist), we demonstrate a distinct excitotoxic sequence of events leading to acute neuronal damage in the hippocampal slice as measured by (1) loss of the evoked CA1 field potential, (2) irreversible changes in light transmittance, (3) histopathology, and (4) lucifer yellow injection of single CA1 pyramidal neurons. Change in light transmittance (LT) through the submerged slice indirectly measures altered cell volume, both neuronal and glial. At 37 degrees C, a 1-min superfusion of 10 mu M domoate induced a prolonged reversible increase in LT, primarily in the dendritic regions of CA1 and dentate granule cells (GC), but not in the CA3 region. Spectral analysis (400-800 nm) revealed a wide-band transmittance increase, indicating cell swelling as a major source of the intrinsic signal. The evoked field potential recorded in the CA1 cell body region (PYR) was lost as LT peaked, but completely recovered upon return to the baseline LT level. Increasing domoate exposure to 10 min elicited a different and distinct LT sequence in CA1 and dentate regions. An initial LT increase in dendritic regions evolved in an irreversible decrease in LT. At the same time, LT irreversibly increased in cell body regions (CA1 PYR and GC) and the evoked field potential was irretrievably lost. Also, there was histological damage to cell body and dendritic regions of CA1 and granule cells. Injection of lucifer yellow into single CA1 neurons in slices displaying the irreversible LT sequence revealed extensive dendritic beading, whereas CA1 cells in control slices displayed a smoothly contoured arbor. Consistent with acute neuronal damage, the optical changes generated by domoate did not require extracellular Ca2+, and lowering the temperature protected the slice from irreversible damage to CA1 and GC regions. Although glial changes may also occur, we conclude that imaging light transmittance reveals dynamic and compartmentalized excitotoxic changes in neuronal volume. Beading of the dendritic arbor increases light scatter, thereby decreasing LT and highlighting damaged dendritic regions.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Apr
pubmed:issn
0969-9961
pubmed:author
pubmed:issnType
Print
pubmed:volume
4
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
423-37
pubmed:dateRevised
2008-11-21
pubmed:meshHeading
pubmed-meshheading:9666481-Animals, pubmed-meshheading:9666481-Calcium Chloride, pubmed-meshheading:9666481-Dendrites, pubmed-meshheading:9666481-Electrophysiology, pubmed-meshheading:9666481-Fluorescent Dyes, pubmed-meshheading:9666481-Hippocampus, pubmed-meshheading:9666481-Image Cytometry, pubmed-meshheading:9666481-Image Processing, Computer-Assisted, pubmed-meshheading:9666481-Isoquinolines, pubmed-meshheading:9666481-Kainic Acid, pubmed-meshheading:9666481-Male, pubmed-meshheading:9666481-Membrane Potentials, pubmed-meshheading:9666481-Microinjections, pubmed-meshheading:9666481-Nerve Degeneration, pubmed-meshheading:9666481-Neuromuscular Depolarizing Agents, pubmed-meshheading:9666481-Neurons, pubmed-meshheading:9666481-Neurotoxins, pubmed-meshheading:9666481-Optics and Photonics, pubmed-meshheading:9666481-Organ Culture Techniques, pubmed-meshheading:9666481-Rats, pubmed-meshheading:9666481-Rats, Sprague-Dawley, pubmed-meshheading:9666481-Receptors, AMPA
pubmed:year
1998
pubmed:articleTitle
Intrinsic optical signaling denoting neuronal damage in response to acute excitotoxic insult by domoic acid in the hippocampal slice.
pubmed:affiliation
Department of Anatomy and Cell Biology, Queen's University, Kingston, Ontario, Canada.
pubmed:publicationType
Journal Article, Research Support, Non-U.S. Gov't