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| Predicate | Object |
|---|---|
| rdf:type | |
| lifeskim:mentions | |
| pubmed:dateCreated |
1998-11-30
|
| pubmed:abstractText |
We used reverse transcriptase-polymerase chain reaction analysis to study the effects of anti-rejection prophylaxis with an anti-interleukin (IL)-2 receptor (IL-2R) monoclonal antibody (BT563) on the allogeneic process by analyzing intragraft IL-2, IL-4, and IL-15 mRNA expression. Analysis showed an association between rejection and intragraft IL-2 mRNA and IL-4 mRNA transcription, whereas IL-15 was constitutively expressed: IL-2, 62% (8/13) during rejection versus 23% (8/35) during immunological quiescence (P < 0.01); IL-4, 69% versus 23% (P < 0.01). BT563 therapy influenced the intragraft mRNA expression of IL-2 and IL-4 but not of IL-15. In endomyocardial biopsies (EMB) showing rejection, mRNA expression of IL-2 was detectable in 40% (2/5) during BT563 treatment versus 75% (6/8) in the absence of BT563; for IL-4, 23% versus 88%, respectively. In contrast, IL-15 mRNA transcription was not affected. Quantitative analysis in rejection EMB showed comparable IL-15 mRNA levels during and after BT563 treatment. This study demonstrates that therapeutic intervention within the IL-2-dependent T-cell activation cascade does not completely prevent rejection. Other cytokines, such as IL-15, may participate in IL-2-independent rejections.
|
| pubmed:language |
eng
|
| pubmed:journal | |
| pubmed:citationSubset |
IM
|
| pubmed:chemical | |
| pubmed:status |
MEDLINE
|
| pubmed:issn |
0934-0874
|
| pubmed:author | |
| pubmed:issnType |
Print
|
| pubmed:volume |
11 Suppl 1
|
| pubmed:owner |
NLM
|
| pubmed:authorsComplete |
Y
|
| pubmed:pagination |
S512-4
|
| pubmed:dateRevised |
2004-11-17
|
| pubmed:meshHeading |
pubmed-meshheading:9665048-Biopsy,
pubmed-meshheading:9665048-Gene Expression,
pubmed-meshheading:9665048-Graft Rejection,
pubmed-meshheading:9665048-Heart Transplantation,
pubmed-meshheading:9665048-Humans,
pubmed-meshheading:9665048-Interleukin-15,
pubmed-meshheading:9665048-Interleukin-2,
pubmed-meshheading:9665048-Interleukin-4,
pubmed-meshheading:9665048-RNA, Messenger
|
| pubmed:year |
1998
|
| pubmed:articleTitle |
Redundancy of the cytokine network in the development of rejection after clinical heart transplantation.
|
| pubmed:affiliation |
Department of Internal Medicine, University Hospital Rotterdam-Dijkzigt, The Netherlands. baan@inw1.azr.nl
|
| pubmed:publicationType |
Journal Article
|