|
rdf:type |
|
|
lifeskim:mentions |
umls-concept:C0007600,
umls-concept:C0007634,
umls-concept:C0011065,
umls-concept:C0018270,
umls-concept:C0021467,
umls-concept:C0021469,
umls-concept:C0033684,
umls-concept:C0085979,
umls-concept:C0086418,
umls-concept:C0179400,
umls-concept:C0812257,
umls-concept:C1414627,
umls-concept:C1417758,
umls-concept:C1419991,
umls-concept:C1514559,
umls-concept:C1546956,
umls-concept:C1707609,
umls-concept:C2347319
|
|
pubmed:issue |
3
|
|
pubmed:dateCreated |
1998-7-29
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|
pubmed:abstractText |
We transfected cells of a guinea pig cell line with RCK cDNA inserted in a pIRES1neo expression vector. The overexpression of rck/p54 was confirmed by Western blot and RT-PCR analysis. In two clones expressing rck/p54, the cell growth was highly inhibited; and their anchorage-independent growth, which is an important character of malignant transformation, was not found. These findings are the first evidence that the overexpression of a DEAD box protein/RNA helicase could inhibit substantially cell growth at the translational level.
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pubmed:commentsCorrections |
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pubmed:language |
eng
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|
pubmed:journal |
|
|
pubmed:citationSubset |
IM
|
|
pubmed:chemical |
|
|
pubmed:status |
MEDLINE
|
|
pubmed:month |
Jun
|
|
pubmed:issn |
0014-5793
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|
pubmed:author |
|
|
pubmed:issnType |
Print
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pubmed:day |
16
|
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pubmed:volume |
429
|
|
pubmed:owner |
NLM
|
|
pubmed:authorsComplete |
Y
|
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pubmed:pagination |
279-83
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|
pubmed:dateRevised |
2009-11-19
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pubmed:meshHeading |
pubmed-meshheading:9662432-Animals,
pubmed-meshheading:9662432-Cell Transformation, Neoplastic,
pubmed-meshheading:9662432-DEAD-box RNA Helicases,
pubmed-meshheading:9662432-Growth Inhibitors,
pubmed-meshheading:9662432-Guinea Pigs,
pubmed-meshheading:9662432-Humans,
pubmed-meshheading:9662432-Proto-Oncogene Proteins,
pubmed-meshheading:9662432-RNA Nucleotidyltransferases,
pubmed-meshheading:9662432-Recombinant Proteins,
pubmed-meshheading:9662432-Transfection,
pubmed-meshheading:9662432-Tumor Cells, Cultured
|
|
pubmed:year |
1998
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|
pubmed:articleTitle |
Growth inhibition by overexpression of human DEAD box protein rck/p54 in cells of a guinea pig cell line.
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|
pubmed:affiliation |
Gifu International Institute of Biotechnology, Yagi Memorial Park, Mitake, Japan. yagiab@po.sphere.ne.jp
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pubmed:publicationType |
Journal Article,
Research Support, Non-U.S. Gov't
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