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rdf:type |
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lifeskim:mentions |
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pubmed:issue |
1
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pubmed:dateCreated |
1998-7-20
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pubmed:abstractText |
Six human cancer cell lines exhibiting a large range of sensitivity to 5-fluorouracil (5-FU) were evaluated for thymidylate synthase (TS) and p53 gene expression, TS and dihydropyrimidine dehydrogenase (DPD) activity, as well as cell cycle parameters, S-phase fraction (SPF), bromodeoxyuridine labelling index (LI) and S-phase duration (SPD). All these parameters were investigated for 7 days in asynchronously growing cell populations and compared with the cell sensitivity to 5-FU. No significant correlation was found between S-phase parameters and TS gene expression and/or activity. TS activity was higher in proliferating cells; however, it was not significantly higher in rapidly growing cell lines with short SPD. Neither TS gene expression nor activity was found to correlate with 5-FU sensitivity. On the another hand, a statistically significant correlation (P < 0.0001) was observed between LI and SPD and 5-FU sensitivity. The present results suggest that cell cycle parameters such as SPD and/or LI could be better parameters for 5-FU sensitivity prediction than TS gene expression and/or activity. This could be especially informative in cases of concomitant radio-chemotherapy as S-phase parameters are already proposed for hyperfractionated radiotherapy planning.
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pubmed:commentsCorrections |
http://linkedlifedata.com/resource/pubmed/commentcorrection/9662252-1695315,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9662252-1704999,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9662252-1924359,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9662252-2293556,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9662252-2402230,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9662252-2514611,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9662252-2987839,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9662252-3029702,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9662252-3155521,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9662252-3312414,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9662252-3584117,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9662252-3843705,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9662252-3943093,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9662252-5545076,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9662252-6162543,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9662252-6396509,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9662252-6713376,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9662252-7497579,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9662252-7779704,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9662252-7833111,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9662252-8261577,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9662252-8608978,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9662252-8869745,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9662252-9062414
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
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pubmed:status |
MEDLINE
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pubmed:month |
Jul
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pubmed:issn |
0007-0920
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pubmed:author |
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pubmed:issnType |
Print
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pubmed:volume |
78
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
62-8
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pubmed:dateRevised |
2009-11-18
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pubmed:meshHeading |
pubmed-meshheading:9662252-Antimetabolites, Antineoplastic,
pubmed-meshheading:9662252-Cell Division,
pubmed-meshheading:9662252-Dihydrouracil Dehydrogenase (NADP),
pubmed-meshheading:9662252-Fluorouracil,
pubmed-meshheading:9662252-Gene Expression Regulation, Enzymologic,
pubmed-meshheading:9662252-Humans,
pubmed-meshheading:9662252-Neoplasm Proteins,
pubmed-meshheading:9662252-Oxidoreductases,
pubmed-meshheading:9662252-S Phase,
pubmed-meshheading:9662252-Thymidylate Synthase,
pubmed-meshheading:9662252-Tumor Cells, Cultured
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pubmed:year |
1998
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pubmed:articleTitle |
Thymidylate synthase expression and activity: relation to S-phase parameters and 5-fluorouracil sensitivity.
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pubmed:affiliation |
Centre Alexis Vautrin, Laboratoire de Recherche en Oncologie, Vandoeuvre-les-Nancy, France.
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pubmed:publicationType |
Journal Article,
Research Support, Non-U.S. Gov't
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