|
rdf:type |
|
|
lifeskim:mentions |
|
|
pubmed:issue |
6
|
|
pubmed:dateCreated |
1998-7-27
|
|
pubmed:abstractText |
In contrast to previous findings in cell-free systems reconstituted with partially purified metazoan factors, we demonstrate dramatic activation of transcription in a TBP-dependent but TAFII-independent manner in HeLa nuclear extracts immunodepleted of TBP and major TAFIIs. Single-round transcription assays reveal that TAFII-independent activation is manifested at the level of productive preinitiation complex formation and that TAFIIs actually impair functional preinitiation complex assembly in a core promoter-specific manner. Furthermore, TAFIIs appear to elevate absolute levels of transcription under multiple-round transcription conditions, presumably by facilitating secondary initiation events. Finally, human coactivator activities related to those in yeast RNA polymerase II/mediator complexes appear to function in unfractionated HeLa nuclear extracts.
|
|
pubmed:language |
eng
|
|
pubmed:journal |
|
|
pubmed:citationSubset |
IM
|
|
pubmed:chemical |
http://linkedlifedata.com/resource/pubmed/chemical/Cell Extracts,
http://linkedlifedata.com/resource/pubmed/chemical/DNA-Binding Proteins,
http://linkedlifedata.com/resource/pubmed/chemical/Fungal Proteins,
http://linkedlifedata.com/resource/pubmed/chemical/Gal-VP16,
http://linkedlifedata.com/resource/pubmed/chemical/Mediator Complex,
http://linkedlifedata.com/resource/pubmed/chemical/Proline,
http://linkedlifedata.com/resource/pubmed/chemical/SRB7 protein, S cerevisiae,
http://linkedlifedata.com/resource/pubmed/chemical/Saccharomyces cerevisiae Proteins,
http://linkedlifedata.com/resource/pubmed/chemical/TAF1 protein, S cerevisiae,
http://linkedlifedata.com/resource/pubmed/chemical/TATA-Binding Protein Associated...,
http://linkedlifedata.com/resource/pubmed/chemical/Trans-Activators,
http://linkedlifedata.com/resource/pubmed/chemical/Transcription Factor TFIID,
http://linkedlifedata.com/resource/pubmed/chemical/Transcription Factors
|
|
pubmed:status |
MEDLINE
|
|
pubmed:month |
May
|
|
pubmed:issn |
1097-2765
|
|
pubmed:author |
|
|
pubmed:issnType |
Print
|
|
pubmed:volume |
1
|
|
pubmed:owner |
NLM
|
|
pubmed:authorsComplete |
Y
|
|
pubmed:pagination |
925-31
|
|
pubmed:dateRevised |
2009-11-19
|
|
pubmed:meshHeading |
pubmed-meshheading:9660976-Cell Extracts,
pubmed-meshheading:9660976-Cell-Free System,
pubmed-meshheading:9660976-DNA-Binding Proteins,
pubmed-meshheading:9660976-Fungal Proteins,
pubmed-meshheading:9660976-HeLa Cells,
pubmed-meshheading:9660976-Humans,
pubmed-meshheading:9660976-Mediator Complex,
pubmed-meshheading:9660976-Mutagenesis,
pubmed-meshheading:9660976-Proline,
pubmed-meshheading:9660976-Promoter Regions, Genetic,
pubmed-meshheading:9660976-Protein Structure, Tertiary,
pubmed-meshheading:9660976-Saccharomyces cerevisiae Proteins,
pubmed-meshheading:9660976-TATA-Binding Protein Associated Factors,
pubmed-meshheading:9660976-Trans-Activators,
pubmed-meshheading:9660976-Transcription Factor TFIID,
pubmed-meshheading:9660976-Transcription Factors,
pubmed-meshheading:9660976-Transcriptional Activation
|
|
pubmed:year |
1998
|
|
pubmed:articleTitle |
Transcription activation via enhanced preinitiation complex assembly in a human cell-free system lacking TAFIIs.
|
|
pubmed:affiliation |
Laboratory of Biochemistry and Molecular Biology, Rockefeller University, New York, New York 10021, USA.
|
|
pubmed:publicationType |
Journal Article,
Research Support, U.S. Gov't, P.H.S.,
Research Support, Non-U.S. Gov't
|
