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rdf:type |
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lifeskim:mentions |
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pubmed:issue |
4
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pubmed:dateCreated |
1998-7-28
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pubmed:abstractText |
We have identified a virus-activated factor (VAF) that binds to a regulatory element shared by different virus-inducible genes. We provide evidence that VAF contains two members of the interferon regulatory factor (IRF) family of transcriptional activator proteins (IRF-3 and IRF-7), as well as the transcriptional coactivator proteins p300 and CBP. Remarkably, VAF, as well as recombinant IRF-3 and IRF-7 proteins, binds very weakly to the interferon-beta (IFN-beta) gene promoter in vitro. However, in virus-infected cells, both proteins are recruited to the endogenous IFN-beta promoter as part of a protein complex that includes ATF-2/c-Jun and NF-kappa B. These observations provide a unique example of the coordinate activation of multiple transcriptional activator proteins and their highly cooperative assembly into a transcriptional enhancer complex in vivo.
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pubmed:grant |
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pubmed:commentsCorrections |
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
http://linkedlifedata.com/resource/pubmed/chemical/ATF2 protein, human,
http://linkedlifedata.com/resource/pubmed/chemical/Activating Transcription Factor 2,
http://linkedlifedata.com/resource/pubmed/chemical/CREB-Binding Protein,
http://linkedlifedata.com/resource/pubmed/chemical/CREBBP protein, human,
http://linkedlifedata.com/resource/pubmed/chemical/Cyclic AMP Response...,
http://linkedlifedata.com/resource/pubmed/chemical/DNA,
http://linkedlifedata.com/resource/pubmed/chemical/DNA-Binding Proteins,
http://linkedlifedata.com/resource/pubmed/chemical/Fungal Proteins,
http://linkedlifedata.com/resource/pubmed/chemical/GAL4 protein, S cerevisiae,
http://linkedlifedata.com/resource/pubmed/chemical/IRF3 protein, human,
http://linkedlifedata.com/resource/pubmed/chemical/IRF7 protein, human,
http://linkedlifedata.com/resource/pubmed/chemical/Interferon Regulatory Factor-3,
http://linkedlifedata.com/resource/pubmed/chemical/Interferon Regulatory Factor-7,
http://linkedlifedata.com/resource/pubmed/chemical/Interferon-beta,
http://linkedlifedata.com/resource/pubmed/chemical/NF-kappa B,
http://linkedlifedata.com/resource/pubmed/chemical/Nuclear Proteins,
http://linkedlifedata.com/resource/pubmed/chemical/Recombinant Fusion Proteins,
http://linkedlifedata.com/resource/pubmed/chemical/Saccharomyces cerevisiae Proteins,
http://linkedlifedata.com/resource/pubmed/chemical/Trans-Activators,
http://linkedlifedata.com/resource/pubmed/chemical/Transcription Factors
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pubmed:status |
MEDLINE
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pubmed:month |
Mar
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pubmed:issn |
1097-2765
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pubmed:author |
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pubmed:issnType |
Print
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pubmed:volume |
1
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
507-18
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pubmed:dateRevised |
2008-11-21
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pubmed:meshHeading |
pubmed-meshheading:9660935-Activating Transcription Factor 2,
pubmed-meshheading:9660935-Animals,
pubmed-meshheading:9660935-Base Sequence,
pubmed-meshheading:9660935-CHO Cells,
pubmed-meshheading:9660935-CREB-Binding Protein,
pubmed-meshheading:9660935-Cell Nucleus,
pubmed-meshheading:9660935-Cricetinae,
pubmed-meshheading:9660935-Cyclic AMP Response Element-Binding Protein,
pubmed-meshheading:9660935-DNA,
pubmed-meshheading:9660935-DNA-Binding Proteins,
pubmed-meshheading:9660935-Enhancer Elements, Genetic,
pubmed-meshheading:9660935-Fungal Proteins,
pubmed-meshheading:9660935-Gene Expression Regulation, Viral,
pubmed-meshheading:9660935-HeLa Cells,
pubmed-meshheading:9660935-Humans,
pubmed-meshheading:9660935-Interferon Regulatory Factor-3,
pubmed-meshheading:9660935-Interferon Regulatory Factor-7,
pubmed-meshheading:9660935-Interferon-beta,
pubmed-meshheading:9660935-Leucine Zippers,
pubmed-meshheading:9660935-Molecular Sequence Data,
pubmed-meshheading:9660935-NF-kappa B,
pubmed-meshheading:9660935-Nuclear Proteins,
pubmed-meshheading:9660935-Phosphorylation,
pubmed-meshheading:9660935-Recombinant Fusion Proteins,
pubmed-meshheading:9660935-Respirovirus,
pubmed-meshheading:9660935-Respirovirus Infections,
pubmed-meshheading:9660935-Saccharomyces cerevisiae Proteins,
pubmed-meshheading:9660935-Trans-Activators,
pubmed-meshheading:9660935-Transcription, Genetic,
pubmed-meshheading:9660935-Transcription Factors
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pubmed:year |
1998
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pubmed:articleTitle |
Virus infection induces the assembly of coordinately activated transcription factors on the IFN-beta enhancer in vivo.
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pubmed:affiliation |
Department of Molecular and Cellular Biology, Harvard University, Cambridge, Massachusetts 02138, USA.
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pubmed:publicationType |
Journal Article,
Research Support, U.S. Gov't, P.H.S.,
Research Support, Non-U.S. Gov't
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