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rdf:type |
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lifeskim:mentions |
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pubmed:issue |
1
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pubmed:dateCreated |
1998-7-24
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pubmed:databankReference |
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pubmed:abstractText |
Exposure of colorectal cancer (CRC) cells to ionizing radiation results in a cell-cycle arrest in G1 and G2. The G1 arrest is due to p53-mediated induction of the cyclin-dependent kinase inhibitor p21WAF1/CIP1/SDI1, but the basis for the G2 arrest is unknown. Through a quantitative analysis of gene expression patterns in CRC cell lines, we have discovered that 14-3-3 sigma is strongly induced by gamma irradiation and other DNA-damaging agents. The induction of 14-3-3 sigma is mediated by a p53-responsive element located 1.8 kb upstream of its transcription start site. Exogenous introduction of 14-3-3 sigma into cycling cells results in a G2 arrest. As the fission yeast 14-3-3 homologs rad24 and rad25 mediate similar checkpoint effects, these results document a molecular mechanism for G2/M control that is conserved throughout eukaryotic evolution and regulated in human cells by p53.
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pubmed:grant |
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
http://linkedlifedata.com/resource/pubmed/chemical/14-3-3 Proteins,
http://linkedlifedata.com/resource/pubmed/chemical/CDKN1A protein, human,
http://linkedlifedata.com/resource/pubmed/chemical/Cyclin-Dependent Kinase Inhibitor...,
http://linkedlifedata.com/resource/pubmed/chemical/Cyclins,
http://linkedlifedata.com/resource/pubmed/chemical/Enzyme Inhibitors,
http://linkedlifedata.com/resource/pubmed/chemical/Exonucleases,
http://linkedlifedata.com/resource/pubmed/chemical/Growth Inhibitors,
http://linkedlifedata.com/resource/pubmed/chemical/Isoenzymes,
http://linkedlifedata.com/resource/pubmed/chemical/Neoplasm Proteins,
http://linkedlifedata.com/resource/pubmed/chemical/Proteins,
http://linkedlifedata.com/resource/pubmed/chemical/RNA, Messenger,
http://linkedlifedata.com/resource/pubmed/chemical/SFN protein, human,
http://linkedlifedata.com/resource/pubmed/chemical/Tumor Markers, Biological,
http://linkedlifedata.com/resource/pubmed/chemical/Tumor Suppressor Protein p53,
http://linkedlifedata.com/resource/pubmed/chemical/Tyrosine 3-Monooxygenase
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pubmed:status |
MEDLINE
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pubmed:month |
Dec
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pubmed:issn |
1097-2765
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pubmed:author |
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pubmed:issnType |
Print
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pubmed:volume |
1
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
3-11
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pubmed:dateRevised |
2007-11-14
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pubmed:meshHeading |
pubmed-meshheading:9659898-14-3-3 Proteins,
pubmed-meshheading:9659898-Colorectal Neoplasms,
pubmed-meshheading:9659898-Cyclin-Dependent Kinase Inhibitor p21,
pubmed-meshheading:9659898-Cyclins,
pubmed-meshheading:9659898-DNA Damage,
pubmed-meshheading:9659898-Enzyme Inhibitors,
pubmed-meshheading:9659898-Eukaryotic Cells,
pubmed-meshheading:9659898-Evolution, Molecular,
pubmed-meshheading:9659898-Exonucleases,
pubmed-meshheading:9659898-G2 Phase,
pubmed-meshheading:9659898-Gene Expression Regulation, Neoplastic,
pubmed-meshheading:9659898-Growth Inhibitors,
pubmed-meshheading:9659898-Humans,
pubmed-meshheading:9659898-In Situ Hybridization, Fluorescence,
pubmed-meshheading:9659898-Isoenzymes,
pubmed-meshheading:9659898-Mitosis,
pubmed-meshheading:9659898-Molecular Sequence Data,
pubmed-meshheading:9659898-Neoplasm Proteins,
pubmed-meshheading:9659898-Polyploidy,
pubmed-meshheading:9659898-Proteins,
pubmed-meshheading:9659898-RNA, Messenger,
pubmed-meshheading:9659898-Tumor Cells, Cultured,
pubmed-meshheading:9659898-Tumor Markers, Biological,
pubmed-meshheading:9659898-Tumor Suppressor Protein p53,
pubmed-meshheading:9659898-Tyrosine 3-Monooxygenase
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pubmed:year |
1997
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pubmed:articleTitle |
14-3-3 sigma is a p53-regulated inhibitor of G2/M progression.
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pubmed:affiliation |
Johns Hopkins Oncology Center, Baltimore, Maryland, USA.
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pubmed:publicationType |
Journal Article,
Research Support, U.S. Gov't, P.H.S.,
Research Support, Non-U.S. Gov't
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