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rdf:type |
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lifeskim:mentions |
umls-concept:C0007600,
umls-concept:C0017262,
umls-concept:C0017428,
umls-concept:C0086418,
umls-concept:C0185117,
umls-concept:C0392756,
umls-concept:C0522498,
umls-concept:C0677626,
umls-concept:C0812204,
umls-concept:C1515926,
umls-concept:C1709130,
umls-concept:C2239176,
umls-concept:C2911684
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pubmed:issue |
3
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pubmed:dateCreated |
1998-9-15
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pubmed:abstractText |
We investigated the expression and genomic alteration of nm23-H1 (which encodes a nucleoside diphosphate, kinase A) in 12 human hepatocellular carcinomas (HCCs) and four hepatoma cell lines. The expression of nm23-H1 protein was significantly reduced in HCCs with intrahepatic metastasis (72%) compared with expression in HCCs without intrahepatic metastasis (38%). However, in two of three HCCs examined that had marked reduction of nm23-H1 protein, the nm23-H1 mRNA level was not reduced. A hepatoma cell line, HLF (phenotype, poorly differentiated carcinoma) revealed marked reduction of nm23-H1 protein compared with two other hepatoma cell lines, HuH-1 and HuH-2, although the mRNA level was similar in the three cell lines. No allelic deletion of the nm23-H1 gene was detected in the 12 HCCs examined. No point mutation in the coding region of the nm23-H1 gene was observed in any of the 12 HCCs or the four hepatoma cell lines. These findings suggest that: (i) the expression of nm23-H1 protein is inversely associated with high metastatic potential of HCC, (ii) regulation of nm23-H1 expression may occasionally occur at both the transcriptional and post-transcriptional levels in HCC; and (iii) genomic alteration of nm23-H1 is a rare event in HCC.
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
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pubmed:status |
MEDLINE
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pubmed:month |
Jun
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pubmed:issn |
0944-1174
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pubmed:author |
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pubmed:issnType |
Print
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pubmed:volume |
33
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
368-75
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pubmed:dateRevised |
2007-11-15
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pubmed:meshHeading |
pubmed-meshheading:9658316-Adult,
pubmed-meshheading:9658316-Aged,
pubmed-meshheading:9658316-Blotting, Northern,
pubmed-meshheading:9658316-Blotting, Western,
pubmed-meshheading:9658316-Carcinoma, Hepatocellular,
pubmed-meshheading:9658316-DNA Primers,
pubmed-meshheading:9658316-Down-Regulation,
pubmed-meshheading:9658316-Exons,
pubmed-meshheading:9658316-Female,
pubmed-meshheading:9658316-Gene Expression Regulation, Neoplastic,
pubmed-meshheading:9658316-Humans,
pubmed-meshheading:9658316-Immunohistochemistry,
pubmed-meshheading:9658316-Liver Neoplasms,
pubmed-meshheading:9658316-Male,
pubmed-meshheading:9658316-Microsatellite Repeats,
pubmed-meshheading:9658316-Middle Aged,
pubmed-meshheading:9658316-Monomeric GTP-Binding Proteins,
pubmed-meshheading:9658316-Mutation,
pubmed-meshheading:9658316-NM23 Nucleoside Diphosphate Kinases,
pubmed-meshheading:9658316-Nucleoside-Diphosphate Kinase,
pubmed-meshheading:9658316-Polymerase Chain Reaction,
pubmed-meshheading:9658316-Polymorphism, Single-Stranded Conformational,
pubmed-meshheading:9658316-Transcription Factors,
pubmed-meshheading:9658316-Tumor Cells, Cultured,
pubmed-meshheading:9658316-Tumor Markers, Biological
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pubmed:year |
1998
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pubmed:articleTitle |
Reduced expression and rare genomic alteration of nm23-H1 in human hepatocellular carcinoma and hepatoma cell lines.
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pubmed:affiliation |
Third Department of Internal Medicine, Asahikawa Medical College, Japan.
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pubmed:publicationType |
Journal Article
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