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Predicate | Object |
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rdf:type | |
lifeskim:mentions | |
pubmed:issue |
1
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pubmed:dateCreated |
1998-7-27
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pubmed:abstractText |
Haemophilus ducreyi causes the genital ulcerative disease chancroid. One putative virulence factor of H. ducreyi is a pore-forming hemolysin that displays toxicity against human fibroblasts and keratinocytes. In order to test the role of the hemolysin in pathogenesis, an isogenic hemolysin-deficient mutant was constructed, designated 35000HP-RSM1. The lipooligosaccharide, outer membrane protein patterns, and growth attributes of 35000HP-RSM1 were identical to its parent, 35000HP. Human subjects were challenged on the upper arm with the isogenic isolates in a double-blinded, randomized, escalating dose-response study. Pustules developed at a similar rate at sites inoculated with the mutant or parent. The cellular infiltrate and bacterial load in lesions were also similar. These results indicate the hemolysin does not play a role in pustule formation. Due to the limitations of this model, the role of the hemolysin at later stages of infection could not be determined.
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pubmed:grant | |
pubmed:language |
eng
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pubmed:journal | |
pubmed:citationSubset |
AIM
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pubmed:chemical | |
pubmed:status |
MEDLINE
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pubmed:month |
Jul
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pubmed:issn |
0022-1899
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pubmed:author | |
pubmed:issnType |
Print
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pubmed:volume |
178
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
191-9
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pubmed:dateRevised |
2007-11-14
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pubmed:meshHeading |
pubmed-meshheading:9652440-Adult,
pubmed-meshheading:9652440-Chancroid,
pubmed-meshheading:9652440-Double-Blind Method,
pubmed-meshheading:9652440-Female,
pubmed-meshheading:9652440-Haemophilus ducreyi,
pubmed-meshheading:9652440-Hemolysin Proteins,
pubmed-meshheading:9652440-Humans,
pubmed-meshheading:9652440-Male,
pubmed-meshheading:9652440-Mutagenesis,
pubmed-meshheading:9652440-Phenotype,
pubmed-meshheading:9652440-Virulence
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pubmed:year |
1998
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pubmed:articleTitle |
Evaluation of an isogenic hemolysin-deficient mutant in the human model of Haemophilus ducreyi infection.
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pubmed:affiliation |
Department of Medicine, Indiana University School of Medicine, Indianapolis 46202, USA.
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pubmed:publicationType |
Journal Article,
Clinical Trial,
Research Support, U.S. Gov't, P.H.S.,
Randomized Controlled Trial
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