Linked Life Data

9652117

Source:http://linkedlifedata.com/resource/pubmed/id/9652117

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
1-2
pubmed:dateCreated
1998-7-20
pubmed:abstractText
An exotoxin, called leukotoxin (LKT), from Pasteurella haemolytica, which had previously proved difficult to purify, was purified by high-performance liquid chromatography using rigid highly hydrophilic microparticulate anion-exchange columns. These anion-exchange stationary phases were employed to overcome difficulties of the relatively hydrophobic LKT interacting with dextran or styrene-based resins. While a short non-porous DEAE column allowed the partial microscale purification of the leukotoxin at pH 7.0, a high capacity strong anion-exchange column of the perfusion chromatography type permitted the purification of LKT on a much larger scale. The purification of the LKT on the large pore strong anion-exchange perfusion column was best achieved when three consecutive linear gradients at increasing NaCl concentration in 20 mM Tris buffer, pH 8.0, containing 6.0 M urea and 0.25% Tween 20 were used. Under these conditions, a better separation was obtained for the tetrameric and aggregate peaks of LKT from the early eluting contaminant peaks. This separation scheme allowed good recovery of activity and purification of the LKT to near homogeneity.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
May
pubmed:issn
0021-9673
pubmed:author
pubmed:issnType
Print
pubmed:day
29
pubmed:volume
808
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
167-76
pubmed:dateRevised
2009-1-15
pubmed:meshHeading
pubmed:year
1998
pubmed:articleTitle
High-performance liquid chromatography of Pasteurella haemolytica leukotoxin using anion-exchange perfusion columns.
pubmed:affiliation
Department of Chemistry, Oklahoma State University, Stillwater 74078-3071, USA.
pubmed:publicationType
Journal Article, Research Support, U.S. Gov't, Non-P.H.S., Research Support, Non-U.S. Gov't