Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
7
pubmed:dateCreated
1998-7-29
pubmed:abstractText
Plants have at least two major photosensory receptors: phytochrome (absorbing primarily red/far-red light) and cryptochrome (absorbing blue/UV-A light); considerable physiological and genetic evidence suggests some form of communication or functional dependence between the receptors. Here, we demonstrate in vitro, using purified recombinant photoreceptors, that Arabidopsis CRY1 and CRY2 (cryptochrome) are substrates for phosphorylation by a phytochrome A-associated kinase activity. Several mutations within the CRY1 C terminus lead to reduced phosphorylation by phytochrome preparations in vitro. Yeast two-hybrid interaction studies using expressed C-terminal fragments of CRY1 and phytochrome A from Arabidopsis confirm a direct physical interaction between both photoreceptors. In vivo labeling studies and specific mutant alleles of CRY1, which interfere with the function of phytochrome, suggest the possible relevance of these findings in vivo.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
http://linkedlifedata.com/resource/pubmed/chemical/Arabidopsis Proteins, http://linkedlifedata.com/resource/pubmed/chemical/CRY1 protein, Arabidopsis, http://linkedlifedata.com/resource/pubmed/chemical/Cryptochromes, http://linkedlifedata.com/resource/pubmed/chemical/Drosophila Proteins, http://linkedlifedata.com/resource/pubmed/chemical/Eye Proteins, http://linkedlifedata.com/resource/pubmed/chemical/Flavoproteins, http://linkedlifedata.com/resource/pubmed/chemical/PHYA protein, Arabidopsis, http://linkedlifedata.com/resource/pubmed/chemical/Phosphates, http://linkedlifedata.com/resource/pubmed/chemical/Phosphotransferases, http://linkedlifedata.com/resource/pubmed/chemical/Phytochrome, http://linkedlifedata.com/resource/pubmed/chemical/Phytochrome A, http://linkedlifedata.com/resource/pubmed/chemical/Plant Proteins, http://linkedlifedata.com/resource/pubmed/chemical/Receptors, G-Protein-Coupled, http://linkedlifedata.com/resource/pubmed/chemical/Recombinant Fusion Proteins, http://linkedlifedata.com/resource/pubmed/chemical/cryptochrome protein, Drosophila
pubmed:status
MEDLINE
pubmed:month
Jun
pubmed:issn
1097-2765
pubmed:author
pubmed:issnType
Print
pubmed:volume
1
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
939-48
pubmed:dateRevised
2009-11-19
pubmed:meshHeading
pubmed-meshheading:9651577-Alleles, pubmed-meshheading:9651577-Arabidopsis, pubmed-meshheading:9651577-Arabidopsis Proteins, pubmed-meshheading:9651577-Cryptochromes, pubmed-meshheading:9651577-Drosophila Proteins, pubmed-meshheading:9651577-Eye Proteins, pubmed-meshheading:9651577-Flavoproteins, pubmed-meshheading:9651577-Mutation, pubmed-meshheading:9651577-Phosphates, pubmed-meshheading:9651577-Phosphorylation, pubmed-meshheading:9651577-Phosphotransferases, pubmed-meshheading:9651577-Photoreceptor Cells, pubmed-meshheading:9651577-Photoreceptor Cells, Invertebrate, pubmed-meshheading:9651577-Phytochrome, pubmed-meshheading:9651577-Phytochrome A, pubmed-meshheading:9651577-Plant Proteins, pubmed-meshheading:9651577-Plants, Genetically Modified, pubmed-meshheading:9651577-Protein Binding, pubmed-meshheading:9651577-Receptors, G-Protein-Coupled, pubmed-meshheading:9651577-Recombinant Fusion Proteins, pubmed-meshheading:9651577-Saccharomyces cerevisiae
pubmed:year
1998
pubmed:articleTitle
The CRY1 blue light photoreceptor of Arabidopsis interacts with phytochrome A in vitro.
pubmed:affiliation
Department of Biology, University of Pennsylvania, Philadelphia 19104-6018, USA. mahmad@sas.upenn.edu
pubmed:publicationType
Journal Article, Research Support, U.S. Gov't, Non-P.H.S.