Switch to
| Predicate | Object |
|---|---|
| rdf:type | |
| lifeskim:mentions | |
| pubmed:issue |
1
|
| pubmed:dateCreated |
1998-8-5
|
| pubmed:abstractText |
Glial cells extrude acid equivalents to maintain pHi. Although four mechanisms have been described so far, pHi-control under physiological conditions is still not sufficiently explained. We therefore investigated whether a H+-translocating ATPase is involved in glial pHi homeostasis using an established glial cell line (C6 glioma). In the absence of bicarbonate, the inhibition of H+-ATPases by NEM led to a pHi decrease. The application of a more specific inhibitor (NBD-Cl) showed that the H+-ATPase involved is of the vacuolar type. Inhibition went along with delayed cell swelling. Together with the fact that glial acidification was far more pronounced in Na+-free media, this may serve as evidence for a secondary activation of Na+/H+-exchange once an activation setpoint is reached, which in turn causes secondary swelling from Na+-uptake. Stimulation of Na+/H+-exchange by PMA can increase the setpoint. pHi-recovery after an acid load was blocked by the inhibition of v-type H+-ATPase, if pHi did not reach 6.6 during the acid load. The inhibition of Na+/H+-exchange by amiloride inhibited recovery only if acidification was below the threshold. Finally, in bicarbonate-free media a v-type H+-ATPase contributes to pH-regulation in glial cells, especially during pH-homeostasis at physiological conditions, while Na+/H+-exchange gains significance during severe acid loads.
|
| pubmed:language |
eng
|
| pubmed:journal | |
| pubmed:citationSubset |
IM
|
| pubmed:chemical | |
| pubmed:status |
MEDLINE
|
| pubmed:month |
Jun
|
| pubmed:issn |
0006-3002
|
| pubmed:author | |
| pubmed:copyrightInfo |
Copyright 1998 Elsevier Science B.V. All rights reserved.
|
| pubmed:issnType |
Print
|
| pubmed:day |
24
|
| pubmed:volume |
1372
|
| pubmed:owner |
NLM
|
| pubmed:authorsComplete |
Y
|
| pubmed:pagination |
28-36
|
| pubmed:dateRevised |
2006-11-15
|
| pubmed:meshHeading |
pubmed-meshheading:9651471-Acids,
pubmed-meshheading:9651471-Animals,
pubmed-meshheading:9651471-Biological Transport,
pubmed-meshheading:9651471-Cell Size,
pubmed-meshheading:9651471-Culture Media,
pubmed-meshheading:9651471-Glioma,
pubmed-meshheading:9651471-Hydrogen-Ion Concentration,
pubmed-meshheading:9651471-Intracellular Fluid,
pubmed-meshheading:9651471-Proton-Translocating ATPases,
pubmed-meshheading:9651471-Protons,
pubmed-meshheading:9651471-Rats,
pubmed-meshheading:9651471-Tumor Cells, Cultured
|
| pubmed:year |
1998
|
| pubmed:articleTitle |
A proton-translocating H+-ATPase is involved in C6 glial pH regulation.
|
| pubmed:affiliation |
Institute for Neurosurgical Pathophysiology, Johannes Gutenberg-University Mainz, 55101 Mainz, Germany.
|
| pubmed:publicationType |
Journal Article,
Research Support, Non-U.S. Gov't
|