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rdf:type |
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lifeskim:mentions |
umls-concept:C0007634,
umls-concept:C0040100,
umls-concept:C0086418,
umls-concept:C0183683,
umls-concept:C0229951,
umls-concept:C0344211,
umls-concept:C0483191,
umls-concept:C1155013,
umls-concept:C1171411,
umls-concept:C1317973,
umls-concept:C1521721,
umls-concept:C1533691,
umls-concept:C1709160
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pubmed:issue |
3
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pubmed:dateCreated |
1998-7-15
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pubmed:abstractText |
Human thymoma is a thymic epithelial cell tumour which often contains a large number of immature T cells and is frequently associated with autoimmune diseases. Since thymic epithelial cells play key roles in the development and selection of T cells in the normal thymus, we hypothesized that the neoplastic thymic epithelial cells of thymoma may support T cell differentiation in the tumour. We characterized CD4-CD8- cells in thymoma and applied an in vitro reconstitution culture system using the CD4-CD8- cells and the neoplastic epithelial cells isolated from thymoma. CD34, a stem cell marker, was expressed on 29.9 +/- 12.2% of CD4-CD8- cells in thymoma. TCRgammadelta was expressed on 27.4 +/- 15.1% of CD4-CD8- cells and CD19, a B cell marker, was expressed on 14.1 +/- 23.1% of CD4-CD8- cells. CD4-CD8- cells expressed both IL-7R alpha-chain and common gamma-chain. Purified CD4-CD8- cells from thymomas were cultured with the neoplastic epithelial cells, and their differentiation into CD4+CD8+ cells via CD4 single-positive intermediates was observed within 9 days' co-culture in the presence of recombinant IL-7. Furthermore, we examined the reconstitution culture using CD34+CD4-CD8- cells purified from normal infant thymus. The CD34+CD4-CD8- cells in normal thymus also differentiated to CD4+CD8+ cells in the allogeneic co-culture with the neoplastic epithelial cells of thymoma. These results indicate that the tumour cells of thymoma retain the function of thymic epithelial cells and can induce differentiation of T cells in thymoma.
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pubmed:commentsCorrections |
http://linkedlifedata.com/resource/pubmed/commentcorrection/9649210-1463094,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9649210-1512547,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9649210-1719121,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9649210-1828260,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9649210-2022918,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9649210-2301282,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9649210-2438939,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9649210-2787360,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9649210-3284290,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9649210-3677008,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9649210-7484464,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9649210-7505122,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9649210-7508157,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9649210-7514905,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9649210-7684429,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9649210-7688021,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9649210-7697728,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9649210-7706715,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9649210-7871393,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9649210-7902570,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9649210-7910843,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9649210-7914216,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9649210-8011206,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9649210-8011212,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9649210-8104713,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9649210-8282311,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9649210-8301114,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9649210-8564838,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9649210-8647189,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9649210-8779719,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9649210-8805634,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9649210-9058787,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9649210-9174611
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
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pubmed:status |
MEDLINE
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pubmed:month |
Jun
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pubmed:issn |
0009-9104
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pubmed:author |
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pubmed:issnType |
Print
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pubmed:volume |
112
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
419-26
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pubmed:dateRevised |
2009-11-18
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pubmed:meshHeading |
pubmed-meshheading:9649210-Adult,
pubmed-meshheading:9649210-Aged,
pubmed-meshheading:9649210-Antigens, CD4,
pubmed-meshheading:9649210-Antigens, CD8,
pubmed-meshheading:9649210-Cell Differentiation,
pubmed-meshheading:9649210-Child, Preschool,
pubmed-meshheading:9649210-Coculture Techniques,
pubmed-meshheading:9649210-Epithelial Cells,
pubmed-meshheading:9649210-Female,
pubmed-meshheading:9649210-Humans,
pubmed-meshheading:9649210-Immunophenotyping,
pubmed-meshheading:9649210-Infant,
pubmed-meshheading:9649210-Male,
pubmed-meshheading:9649210-Middle Aged,
pubmed-meshheading:9649210-Receptors, Antigen, T-Cell, gamma-delta,
pubmed-meshheading:9649210-Stromal Cells,
pubmed-meshheading:9649210-T-Lymphocytes,
pubmed-meshheading:9649210-Thymoma,
pubmed-meshheading:9649210-Tumor Cells, Cultured
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pubmed:year |
1998
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pubmed:articleTitle |
Neoplastic thymic epithelial cells of human thymoma support T cell development from CD4-CD8- cells to CD4+CD8+ cells in vitro.
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pubmed:affiliation |
First Department of Surgery, Osaka University Medical School, Suita, Japan.
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pubmed:publicationType |
Journal Article
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