Linked Life Data

9648886

Source:http://linkedlifedata.com/resource/pubmed/id/9648886

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
1
pubmed:dateCreated
1998-7-9
pubmed:abstractText
Ceramide generated from sphingomyelin has emerged as a new but conserved type of biologically active lipid. We previously found that endogenous sphingolipids are required for the normal growth of cultured cerebellar Purkinje neurons and that sphingomyelin is present abundantly in the somatodendritic region of these cells. To gain further insight into a potential role of the sphingomyelin/ceramide pathway, we investigated the effects of depletion of sphingolipids on the phenotypic growth and survival of immature Purkinje cells and the ability of ceramide or other sphingolipids to antagonize these effects. Inhibition of ceramide synthesis by ISP-1, a specific inhibitor of serine palmitoyltransferase, decreased cellular levels of sphingolipids. This treatment resulted in a decrease in cell survival accompanied by an induction of apoptotic cell death and aberrant dendritic differentiation of Purkinje cells with no detectable changes in other cerebellar neurons. Cell-permeable ceramides, sphingosine, or sphingomyelin overcame these abnormalities more effectively than other sphingolipids when added simultaneously with ISP-1. Exposure to bacterial sphingomyelinase in turn enhanced cell survival and dendritic branching complexity of Purkinje cells at different optimal concentrations. Furthermore, cell-permeable ceramide acted synergistically with the neurotrophin family, which has been previously shown to support Purkinje cell survival. These observations suggest that ceramide is a requisite for the survival and the dendritic differentiation of Purkinje cells.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Jul
pubmed:issn
0022-3042
pubmed:author
pubmed:issnType
Print
pubmed:volume
71
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
366-77
pubmed:dateRevised
2006-11-15
pubmed:meshHeading
pubmed-meshheading:9648886-Animals, pubmed-meshheading:9648886-Apoptosis, pubmed-meshheading:9648886-Cell Differentiation, pubmed-meshheading:9648886-Cell Membrane Permeability, pubmed-meshheading:9648886-Cell Size, pubmed-meshheading:9648886-Cell Survival, pubmed-meshheading:9648886-Cells, Cultured, pubmed-meshheading:9648886-Ceramides, pubmed-meshheading:9648886-Dendrites, pubmed-meshheading:9648886-Drug Synergism, pubmed-meshheading:9648886-Fatty Acids, Monounsaturated, pubmed-meshheading:9648886-Fetus, pubmed-meshheading:9648886-Immunosuppressive Agents, pubmed-meshheading:9648886-Nerve Growth Factors, pubmed-meshheading:9648886-Neurotrophin 3, pubmed-meshheading:9648886-Purkinje Cells, pubmed-meshheading:9648886-Rats, pubmed-meshheading:9648886-Rats, Wistar, pubmed-meshheading:9648886-Sphingomyelin Phosphodiesterase, pubmed-meshheading:9648886-Sphingosine, pubmed-meshheading:9648886-Tritium
pubmed:year
1998
pubmed:articleTitle
Ceramide and its interconvertible metabolite sphingosine function as indispensable lipid factors involved in survival and dendritic differentiation of cerebellar Purkinje cells.
pubmed:affiliation
Laboratory for Cellular Glycobiology, Frontier Research Program, Institute of Physical and Chemical Research (RIKEN), Wako, Saitama, Japan.
pubmed:publicationType
Journal Article, Research Support, Non-U.S. Gov't