Linked Life Data

9648723

Source:http://linkedlifedata.com/resource/pubmed/id/9648723

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
12
pubmed:dateCreated
1998-7-20
pubmed:abstractText
Angiotensin II (Ang II) has been previously shown to stimulate the extracellular signal-regulated kinase (ERK) 1/2 and c-Jun N-terminal kinase (JNK) mitogen-activated protein (MAP) kinase family members. Little is known regarding the upstream signaling molecules involved in Ang II-mediated JNK activation. Ang II has been shown to activate the Janus kinase/signal transducer(s) and activator(s) of transcription (JAK/STAT) pathway, suggesting similarities to cytokine signaling. In response to cytokines such as interleukin-1 and tumor necrosis factor-alpha, the p21-activated kinase (PAK) has been identified as an upstream component in JNK activation. Therefore, we hypothesized that PAK may be involved in JNK activation by Ang II in vascular smooth muscle cells (VSMCs). AlphaPAK activity was measured by myelin basic protein phosphorylation in rat aortic VSMCs. In response to Ang II, alphaPAK was rapidly stimulated within 1 minute, with a peak (5-fold increase) at 30 minutes. AlphaPAK stimulation preceded activation of JNK in VSMCs. Ang II-mediated activation of both alphaPAK and JNK was Ca2+ dependent and inhibited by downregulation of phorbol ester-sensitive protein kinase C isoforms (by pretreatment with phorbol 12,13-dibutyrate) but not by pretreatment with GF109203X. Activation of both PAK and JNK was partially inhibited by tyrosine kinase inhibitors but not by specific Src inhibitors, suggesting regulation by a tyrosine kinase other than c-Src. Finally, introduction of dominant negative PAK markedly reduced the JNK activation by Ang II in both Chinese hamster ovary and COS cells stably expressing the Ang II type 1 receptor (AT1R). Our data provide evidence for alphaPAK as an upstream mediator of JNK in Ang II signaling and extend the role of Ang II as a proinflammatory mediator for VSMCs.
pubmed:grant
pubmed:commentsCorrections
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Jun
pubmed:issn
0009-7330
pubmed:author
pubmed:issnType
Print
pubmed:day
29
pubmed:volume
82
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
1272-8
pubmed:dateRevised
2009-11-19
pubmed:meshHeading
pubmed-meshheading:9648723-Angiotensin II, pubmed-meshheading:9648723-Animals, pubmed-meshheading:9648723-Calcium, pubmed-meshheading:9648723-Calcium-Calmodulin-Dependent Protein Kinases, pubmed-meshheading:9648723-Cells, Cultured, pubmed-meshheading:9648723-Cricetinae, pubmed-meshheading:9648723-Down-Regulation, pubmed-meshheading:9648723-Enzyme Activation, pubmed-meshheading:9648723-Isoenzymes, pubmed-meshheading:9648723-JNK Mitogen-Activated Protein Kinases, pubmed-meshheading:9648723-Mitogen-Activated Protein Kinases, pubmed-meshheading:9648723-Muscle, Smooth, Vascular, pubmed-meshheading:9648723-Protein Kinase C, pubmed-meshheading:9648723-Protein-Serine-Threonine Kinases, pubmed-meshheading:9648723-Rats, pubmed-meshheading:9648723-Signal Transduction, pubmed-meshheading:9648723-p21-Activated Kinases
pubmed:year
1998
pubmed:articleTitle
Angiotensin II stimulates p21-activated kinase in vascular smooth muscle cells: role in activation of JNK.
pubmed:affiliation
Department of Medicine, University of Washington, Seattle 98195, USA.
pubmed:publicationType
Journal Article, Research Support, U.S. Gov't, P.H.S., Research Support, Non-U.S. Gov't