Linked Life Data

9647696

Source:http://linkedlifedata.com/resource/pubmed/id/9647696

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
1
pubmed:dateCreated
1998-8-11
pubmed:abstractText
Sequential observations were made of the ultrastructural effects of Bacillus thuringiensis (Bt) subsp. kurstaki strain WB3S16 on midgut epithelial cells of the sheep biting louse, Bovicola ovis, after the lice were fed, ad libitum, a powdered preparation of Bt spores, delta-endotoxin crystals, and lysed cellular components. Light microscope observations revealed cytopathological changes to the midgut epithelial cells 4 h postfeeding. Transmission electron micrographs showed that the microvilli of the midgut epithelial cells became disrupted 4-8 h postfeeding, after which the cells became vacuolated and swollen, and the cell organelles lost definition and disappeared. Paralysis and death of B. ovis occurred between 8 and 12 h postfeeding, coincident with midgut cells lysis and release of cellular contents into the midgut lumen. The histopathological effects reported here are similar to those reported in lepidopteran and coleopteran larvae affected by the delta-endotoxin crystal proteins. The constituent fractions of the Bt preparation were tested for toxicity to B. ovis using a feeding bioassay. Native delta-endotoxin crystals were not toxic to B. ovis and remained intact in the midgut of the insect. There was no evidence that the native Bt crystal was involved in the cytopathology and death of the lice. However, in vitro solubilized delta-endotoxin crystal proteins were significantly toxic to B. ovis. In addition, a louse active toxin was associated with the Bt membranes and culture supernatant.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Jul
pubmed:issn
0022-2011
pubmed:author
pubmed:copyrightInfo
Copyright 1998 Academic Press.
pubmed:issnType
Print
pubmed:volume
72
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
9-20
pubmed:dateRevised
2010-11-18
pubmed:meshHeading
pubmed:year
1998
pubmed:articleTitle
Histopathological effects of Bacillus thuringiensis on the alimentary canal of the sheep louse, Bovicola ovis.
pubmed:affiliation
Department of Crop Protection, University of Adelaide, Waite Campus, Glen Osmond, South Australia, 5064, Australia.
pubmed:publicationType
Journal Article, Research Support, Non-U.S. Gov't