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| Predicate | Object |
|---|---|
| rdf:type | |
| lifeskim:mentions | |
| pubmed:issue |
1
|
| pubmed:dateCreated |
1998-7-9
|
| pubmed:abstractText |
We wondered whether the apoptosis known to occur after UV-B irradiation might involve the Fas/Fas ligand (FasL) signaling pathway. We exposed PBLs from normal individuals, and also the Jurkat (E6-1) and U937 cell lines, to graded doses of UV-B irradiation and observed a prompt and marked increase in Fas expression at doses as low as 0.5 mJ/cm2. Increased Fas expression did not require new protein synthesis, since cycloheximide-treated cells also showed an increase in Fas after UV-B. UV-B-irradiated cells cultured in the presence of zinc showed inhibition of apoptosis coincident with a marked increase in Fas+ cells, apparently indicating the accumulation of Fas-bearing cells unable to undergo apoptosis. After UV-B irradiation, PBLs showed increased expression of Fas ligand; the E6-1 lymphocytic cell line also released soluble FasL. UV-B induced apoptosis could be partially blocked by neutralizing FasL Abs, and a FasL-resistant variant of E6-1 cell line showed reduced apoptosis after UV-B irradiation, implying that the increase in Fas expression signified a role for Fas in UV-induced apoptosis. UV-induced Fas expression may serve to target stress-injured cells for removal by FasL-bearing cells or by FasL produced by the cells themselves in response to the stimuli, and may represent a general function of the Fas/FasL pathway in facilitating the apoptosis and elimination of undesirable or harmful cells.
|
| pubmed:grant | |
| pubmed:language |
eng
|
| pubmed:journal | |
| pubmed:citationSubset |
AIM
|
| pubmed:chemical |
http://linkedlifedata.com/resource/pubmed/chemical/Antibodies, Monoclonal,
http://linkedlifedata.com/resource/pubmed/chemical/Antigens, CD95,
http://linkedlifedata.com/resource/pubmed/chemical/Cycloheximide,
http://linkedlifedata.com/resource/pubmed/chemical/FASLG protein, human,
http://linkedlifedata.com/resource/pubmed/chemical/Fas Ligand Protein,
http://linkedlifedata.com/resource/pubmed/chemical/Immune Sera,
http://linkedlifedata.com/resource/pubmed/chemical/Ligands,
http://linkedlifedata.com/resource/pubmed/chemical/Membrane Glycoproteins,
http://linkedlifedata.com/resource/pubmed/chemical/Zinc
|
| pubmed:status |
MEDLINE
|
| pubmed:month |
Jul
|
| pubmed:issn |
0022-1767
|
| pubmed:author | |
| pubmed:issnType |
Print
|
| pubmed:day |
1
|
| pubmed:volume |
161
|
| pubmed:owner |
NLM
|
| pubmed:authorsComplete |
Y
|
| pubmed:pagination |
241-51
|
| pubmed:dateRevised |
2008-11-21
|
| pubmed:meshHeading |
pubmed-meshheading:9647230-Adult,
pubmed-meshheading:9647230-Antibodies, Monoclonal,
pubmed-meshheading:9647230-Antigens, CD95,
pubmed-meshheading:9647230-Apoptosis,
pubmed-meshheading:9647230-Cell Survival,
pubmed-meshheading:9647230-Cells, Cultured,
pubmed-meshheading:9647230-Cycloheximide,
pubmed-meshheading:9647230-Fas Ligand Protein,
pubmed-meshheading:9647230-Humans,
pubmed-meshheading:9647230-Immune Sera,
pubmed-meshheading:9647230-Immunity, Innate,
pubmed-meshheading:9647230-Jurkat Cells,
pubmed-meshheading:9647230-Ligands,
pubmed-meshheading:9647230-Lymphocytes,
pubmed-meshheading:9647230-Lymphoma, Large B-Cell, Diffuse,
pubmed-meshheading:9647230-Membrane Glycoproteins,
pubmed-meshheading:9647230-Solubility,
pubmed-meshheading:9647230-Tumor Cells, Cultured,
pubmed-meshheading:9647230-Ultraviolet Rays,
pubmed-meshheading:9647230-Zinc
|
| pubmed:year |
1998
|
| pubmed:articleTitle |
Fas/Fas ligand interactions are involved in ultraviolet-B-induced human lymphocyte apoptosis.
|
| pubmed:affiliation |
Department of Medicine, University of North Carolina at Chapel Hill 27599, USA.
|
| pubmed:publicationType |
Journal Article,
Research Support, U.S. Gov't, P.H.S.,
Research Support, Non-U.S. Gov't
|