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| Predicate | Object |
|---|---|
| rdf:type | |
| lifeskim:mentions | |
| pubmed:issue |
1
|
| pubmed:dateCreated |
1998-7-9
|
| pubmed:abstractText |
Previous studies have shown that CD8 beta plays a role in both enhancing CD8 alpha-associated Lck kinase activity and promoting the development of CD8-lineage T cells. To examine the role of this enhancement in the maturation of CD8-lineage cells, we assessed CD8 alpha-associated Lck kinase activity in both T cell hybridomas and thymocytes of mice expressing CD8 beta mutations known to impair CD8 T cell development. Lack of CD8 beta expression or expression of a cytoplasmic domain-deleted CD8 beta resulted in a severalfold reduction in CD8 alpha-associated Lck kinase activity compared with that observed with cells expressing wild-type CD8 beta chain. This analysis indicated a critical role for the cytoplasmic domain of CD8 beta in the regulation of CD8 alpha-associated Lck activity. Decreased CD8 alpha-associated Lck activity observed with the various CD8 beta mutations also correlated with diminished in vivo cellular tyrosine phosphorylation. In addition, analysis of CD8 beta mutant mice (CD8 beta-/- or cytoplasmic domain-deleted CD8 beta transgenic) indicated that the degree of reduction in CD8 alpha-associated Lck activity associated with each mutation correlated with the severity of developmental impairment. These results support the importance of CD8 beta-mediated enhancement of CD8 alpha-associated Lck kinase activity in the differentiation of CD8 single-positive thymocytes.
|
| pubmed:grant | |
| pubmed:language |
eng
|
| pubmed:journal | |
| pubmed:citationSubset |
AIM
|
| pubmed:chemical | |
| pubmed:status |
MEDLINE
|
| pubmed:month |
Jul
|
| pubmed:issn |
0022-1767
|
| pubmed:author | |
| pubmed:issnType |
Print
|
| pubmed:day |
1
|
| pubmed:volume |
161
|
| pubmed:owner |
NLM
|
| pubmed:authorsComplete |
Y
|
| pubmed:pagination |
183-91
|
| pubmed:dateRevised |
2009-11-19
|
| pubmed:meshHeading |
pubmed-meshheading:9647223-Animals,
pubmed-meshheading:9647223-Antigens, CD8,
pubmed-meshheading:9647223-CD8-Positive T-Lymphocytes,
pubmed-meshheading:9647223-Cell Differentiation,
pubmed-meshheading:9647223-Cell Line,
pubmed-meshheading:9647223-Cytoplasm,
pubmed-meshheading:9647223-Enzyme Activation,
pubmed-meshheading:9647223-Genetic Vectors,
pubmed-meshheading:9647223-Humans,
pubmed-meshheading:9647223-Hybridomas,
pubmed-meshheading:9647223-Intracellular Fluid,
pubmed-meshheading:9647223-Lymphocyte Specific Protein Tyrosine Kinase p56(lck),
pubmed-meshheading:9647223-Mice,
pubmed-meshheading:9647223-Mice, Knockout,
pubmed-meshheading:9647223-Mice, Transgenic,
pubmed-meshheading:9647223-Phosphorylation,
pubmed-meshheading:9647223-Protein Structure, Tertiary,
pubmed-meshheading:9647223-Sequence Deletion,
pubmed-meshheading:9647223-Substrate Specificity,
pubmed-meshheading:9647223-Tyrosine
|
| pubmed:year |
1998
|
| pubmed:articleTitle |
The cytoplasmic domain of CD8 beta regulates Lck kinase activation and CD8 T cell development.
|
| pubmed:affiliation |
Department of Pediatric Oncology, Dana-Farber Cancer Institute, Harvard Medical School, Boston, MA 02115, USA.
|
| pubmed:publicationType |
Journal Article,
Comparative Study,
Research Support, U.S. Gov't, P.H.S.
|