Switch to
| Predicate | Object |
|---|---|
| rdf:type | |
| lifeskim:mentions | |
| pubmed:issue |
1
|
| pubmed:dateCreated |
1998-7-9
|
| pubmed:abstractText |
In this study, the phenotype, TCR signaling events, and function of T cells developed de novo during adulthood in the presence of extrathymic alloantigen were investigated. C57BL/6 mice(H-2b) were first transplanted heterotopically with BALB/c hearts (H-2d) and treated with rapamycin for 2 wk to create a tolerant status. Three weeks postoperation, the mice were whole body irradiated and transplanted with bone marrow cells from 2C mice, which are transgenic for TCR, and most of their T cells are Ld-specific CD8 cells. The 2C T cells developed de novo in the C57BL/6 mice were not able to reject the heart allograft. No clonal deletion, TCR down-regulation, or CD8 down-regulation was found in the tolerized 2C T cells. There was no characteristic phenotype of these cells in terms of CD25, ICAM-1, CD44, and MEL-14 expression. Early TCR signaling events such as intracellular calcium concentration flux, tyrosine phosphorylation, Lck and Fyn kinase activities, and Lck and Fyn protein levels in the tolerized 2C T cells were comparable to their normal counterparts, but the tolerized T cells were defective in IL-2 production and proliferation upon H-2d alloantigen stimulation in vitro. Exogenous IL-2 could not reverse the compromised proliferation. The results of this study indicate that during adulthood, the de novo-developed T cells become tolerant to extrathymic Ag without clonal deletion. These newly minted T cells are functionally defective although they are indistinguishable from normal T cells in phenotypes and in some early signaling events.
|
| pubmed:language |
eng
|
| pubmed:journal | |
| pubmed:citationSubset |
AIM
|
| pubmed:chemical |
http://linkedlifedata.com/resource/pubmed/chemical/Antigens, CD44,
http://linkedlifedata.com/resource/pubmed/chemical/Epitopes, T-Lymphocyte,
http://linkedlifedata.com/resource/pubmed/chemical/H-2 Antigens,
http://linkedlifedata.com/resource/pubmed/chemical/Intercellular Adhesion Molecule-1,
http://linkedlifedata.com/resource/pubmed/chemical/Interleukin-2,
http://linkedlifedata.com/resource/pubmed/chemical/Isoantigens,
http://linkedlifedata.com/resource/pubmed/chemical/Receptors, Interleukin-2
|
| pubmed:status |
MEDLINE
|
| pubmed:month |
Jul
|
| pubmed:issn |
0022-1767
|
| pubmed:author | |
| pubmed:issnType |
Print
|
| pubmed:day |
1
|
| pubmed:volume |
161
|
| pubmed:owner |
NLM
|
| pubmed:authorsComplete |
Y
|
| pubmed:pagination |
73-82
|
| pubmed:dateRevised |
2006-11-15
|
| pubmed:meshHeading |
pubmed-meshheading:9647209-Animals,
pubmed-meshheading:9647209-Antigens, CD44,
pubmed-meshheading:9647209-Bone Marrow Transplantation,
pubmed-meshheading:9647209-Cell Differentiation,
pubmed-meshheading:9647209-Epitopes, T-Lymphocyte,
pubmed-meshheading:9647209-H-2 Antigens,
pubmed-meshheading:9647209-Heart Transplantation,
pubmed-meshheading:9647209-Immune Tolerance,
pubmed-meshheading:9647209-Immunocompromised Host,
pubmed-meshheading:9647209-Intercellular Adhesion Molecule-1,
pubmed-meshheading:9647209-Interleukin-2,
pubmed-meshheading:9647209-Isoantigens,
pubmed-meshheading:9647209-Lymphocyte Activation,
pubmed-meshheading:9647209-Male,
pubmed-meshheading:9647209-Mice,
pubmed-meshheading:9647209-Mice, Inbred BALB C,
pubmed-meshheading:9647209-Mice, Inbred C57BL,
pubmed-meshheading:9647209-Mice, Transgenic,
pubmed-meshheading:9647209-Radiation Chimera,
pubmed-meshheading:9647209-Receptors, Interleukin-2,
pubmed-meshheading:9647209-Signal Transduction,
pubmed-meshheading:9647209-T-Lymphocytes
|
| pubmed:year |
1998
|
| pubmed:articleTitle |
De novo-developed T cells have compromised response to existing alloantigens: using Ld-specific transgenic 2C T cells as tracers in a mouse heart transplantation model.
|
| pubmed:affiliation |
The Louis-Charles Simard Research Center, Notre-Dame Hospital, CHUM, University of Montreal, Quebec, Canada.
|
| pubmed:publicationType |
Journal Article,
Research Support, Non-U.S. Gov't
|